Aksiniya Asenova University of California, Davis
15 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
51 protocols

Amey Redkar Department of Genetics, University of Cordoba
22 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
17 protocols

Editor
Manjula Mummadisetti
  • Post-Doc, Rutgers university
Research focus
  • Biochemistry
  • Photosynthesis, Plant Biochemistry, Protein Chemistry, Biofuels, Marine Biology and Environmental Sciences, Biomineralization.

Education

PhD, Louisiana State University, 2016

Lab information

mmpdsetti@gmail.com

Publications

4 Protocols reviewed
Biomechanical Characterization of Onion Epidermal Cell Walls
Authors:  Daniel M. Durachko, Yong Bum Park, Tian Zhang and Daniel J. Cosgrove, date: 12/20/2017, view: 9472, Q&A: 1
Here we describe two experimental protocols to measure the biomechanical properties of primary (growing) plant cell walls, with a focus on analyzing cell wall epidermal strips of onion scales. The first protocol measures cell wall creep ...
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A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions
In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum, we supplied 32P to mycelia previously grown in vitro in liquid medium. The culture had four main steps ...
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9 Protocols edited
Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein
Authors:  Bhanu P. Jagilinki, Irina Paluy, Vikas Nanda and Dror Noy, date: 09/20/2021, view: 479, Q&A: 0

Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as

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Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
Authors:  Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim, date: 09/05/2021, view: 1020, Q&A: 0

Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a

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