Nicole Koropatkin Department of Microbiology and Immunology, University of Michigan Medical School, USA
1 protocol
Alexandros Alexandratos National and Kapodistrian University of Athens
64 protocols

Dennis Nürnberg Freie Universität Berlin
51 protocols

Gunjan Mehta National Institutes of Health
7 protocols

Joana Antunes Verogen, Inc
3 protocols

Reviewer
Darrell Cockburn
  • Assistant professor, Pennsylvania State University
Research focus
  • Microbiology
  • Resistant Starch; Gut Microbiome; Carbohydrate Active Enzymes
  • 1 Author merit
1 Protocol published
Product Analysis of Starch Active Enzymes by TLC
Authors:  Darrell Cockburn and Nicole Koropatkin, date: 10/20/2015, view: 11736, Q&A: 1
Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the ...
10 Protocols reviewed
Activity-based Crosslinking to Identify Substrates of Thioredoxin-domain Proteins in Malaria Parasites
Authors:  David W. Cobb, Grace S. Woods and Vasant Muralidharan, date: 02/20/2022, view: 1045, Q&A: 0
Malaria remains a major public health issue, infecting nearly 220 million people every year. The spread of drug-resistant strains of Plasmodium falciparum around the world threatens the progress made against this disease. Therefore, ...
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Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production
Authors:  Patrick J. Shilling and Daniel O. Daley, date: 08/20/2021, view: 5004, Q&A: 0

pET expression plasmids are widely used in the biotechnology, biopharmaceutical, and basic research sectors for the production of recombinant proteins. Typically, they are used off-the-shelf because they support high production titers; however, we

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