Nicole Koropatkin Department of Microbiology and Immunology, University of Michigan Medical School, USA
1 protocol
Dennis Nürnberg Freie Universität Berlin
48 protocols

Gunjan Mehta National Institutes of Health
7 protocols

Joana Antunes Florida International University
3 protocols

Kristin Shingler University of Minnesota School of Dentistry
29 protocols

Reviewer
Darrell Cockburn
  • Assistant professor, Pennsylvania State University
Research focus
  • Microbiology
  • Resistant Starch; Gut Microbiome; Carbohydrate Active Enzymes
  • 1 Author merit
1 Protocol published
Product Analysis of Starch Active Enzymes by TLC
Authors:  Darrell Cockburn and Nicole Koropatkin, date: 10/20/2015, view: 10959, Q&A: 1
Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the ...
9 Protocols reviewed
Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production
Authors:  Patrick J. Shilling and Daniel O. Daley, date: 08/20/2021, view: 1492, Q&A: 0

pET expression plasmids are widely used in the biotechnology, biopharmaceutical, and basic research sectors for the production of recombinant proteins. Typically, they are used off-the-shelf because they support high production titers; however, we

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Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
Authors:  Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty, date: 11/05/2020, view: 1433, Q&A: 0

This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can

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