Agnieszka Zienkiewicz Georg August University
16 protocols

Alba Blesa Universidad Francisco de Vitoria
18 protocols

Alberto Rissone National Institutes of Health
2 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
14 protocols

Editor
David A. Cisneros
  • Research scientist, Umeå University
Research focus
  • Microbiology
  • Microbiology
    Molecular Biology
    Anaerobic microorganisms
    Bacterial Secretion
    CRISPR effectors
    Gut Microbiome
  • 0 Author merit

Education

Dr., TU-Dresden, Germany

Lab information

"Molecular biology of human bacterial symbionts"

The intestinal microbiome consists of trillions of beneficial bacteria, which live harmoniously and synergistically with their human hosts. It has only recently been discovered that such microorganisms enormously impact our health in ways that had been completely unanticipated. Because of this, it has become important to understand how this ‘microbiome’ is built and maintained within the intestines, which is where most of our bacteria live. That is the focus of our work.
https://www.cisneros-lab.org/

Publications

http://scholar.google.com/citations?user=WGDppOUAAAAJ&hl=en
Selected Publications:

*Wutz G., *Várnai C., *Nagasaka K. , *Cisneros D.A., Stocsits R.R., Tang W., Schoenfelder S., Jessberger G., Muhar M., Hossain M.J., Walther N., Koch B., Kueblbeck M., Ellenberg J., Zuber J., Fraser P., Peters J.M (2017). Topologically associating domains and chromatin loops depend on cohesin and are regulatedby CTCF, WAPL, and PDS5 proteins. EMBO J. 36(24), 3573-3599.
*SHARED FIRST AUTHOR.

Davidson, I. F., Goetz, D., Zaczek, M. P., Molodtsov, M. I., in't Veld, P. J. H., Weissmann, F., Litos, G., Cisneros, D. A., Ocampo-Hafalla, M., Ladurner, R., Uhlmann, F., Vaziri, A. and Peters, J. M. (2016). Rapid movement and transcriptional re-localization of human cohesin on DNA. Embo Journal 35, 2671-2685.

Ladurner, R., Kreidl, E., Ivanov, M. P., Ekker, H., Idarraga-Amado, M. H., Busslinger, G. A., Wutz, G., Cisneros, D. A. and Peters, J. M. (2016) Sororin actively maintains sister chromatid cohesion. EMBO Journal 35, pp. 635-653.

Tedeschi, A., Wutz, G., Huet, S.b., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I.F., Tang, W., Cisneros, D.A., Bhaskara, V., Nishiyama T., Vaziri A., Wutz A., Ellenberg J., Peters J.M. (2013). Wapl is an essential regulator of chromatin structure and chromosome segregation. Nature 501, 564-568.

Cisneros, D.A., Pehau-Arnaudet, G., and Francetic, O. (2012). Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation. Molecular microbiology 86, 805-818.

Cisneros, D.A., Bond, P.J., Pugsley, A.P., Campos, M., and Francetic, O. (2012). Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation. The EMBO journal 31, 1041-1053.

Campos, M., Nilges, M.l., Cisneros, D.A., and Francetic, O. (2010). Detailed structural and assembly model of the type II secretion pilus from sparse data. Proceedings of the National Academy of Sciences 107, 13081-13086.

Cisneros, D.A., Muller, D.J., Daud, S.M., and Lakey, J.H. (2006). An Approach To Prepare Membrane Proteins for Single Molecule Imaging. Angewandte Chemie International Edition 45, 3252-3256.

Cisneros, D.A., Hung, C., Franz, C.M., and Muller, D.J. (2006). Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy. Journal of structural biology 154, 232-245
4 Protocols reviewed
Assessment of Metacaspase Activity in Phytoplankton
Authors:  Dina Spungin and Ilana Berman-Frank, date: 08/20/2019, view: 1859, Q&A: 0
Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein ...
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Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
Authors:  Magdiel Martínez, Namyr A. Martínez and Walter I. Silva, date: 07/20/2017, view: 22477, Q&A: 1
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; ...
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27 Protocols edited
Radioactive Assay of in vitro Glutamylation Activity of the Legionella pneumophila Effector Protein SidJ
Authors:  Alan G. Sulpizio, Jung-Ho Shin, Marena E. Minelli and Yuxin Mao, date: 10/05/2020, view: 300, Q&A: 0
The Legionella effector protein SidJ has recently been identified to perform polyglutamylation on another Legionella effector, SdeA, ablating SdeA’s activity. SidJ is a kinase-like protein that requires the small eukaryotic protein ...
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In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes
Authors:  Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama, date: 05/20/2020, view: 1365, Q&A: 0
It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a ...
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