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Veronica Truniger
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol
Miguel Aranda
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol
Jelena Kraft
Department of Plant Pathology and Microbiology, Iowa State University, USA
1 protocol
Raquel Sempere
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol
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W. Allen Miller
Department of Plant Pathology and Microbiology, Iowa State University, USA
Research focus
Microbiology
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1 Protocol published
Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
Authors:
Manuel Miras
,
Raquel N. Sempere
,
Jelena J. Kraft
,
W. Allen Miller
,
Miguel A. Aranda
and
Veronica Truniger
,
date:
01/20/2015,
view:
12282,
Q&A:
0
This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical ...
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