Dear,
Thanks a lot for your question. I am very pleasure to share my opinions.
From your statement, I think your cells are not purified and most of the cells are blood cells (leucocyte). When you culture these cells, are they attached to the plates and spread well?
When I isolated stem cells from cord, adipose and placenta tissues, I usually seed around 10 million cells in a 10 cm plate. Then I remove at least half of the suspension after 24 hours and add the same volume of fresh medium. After that, I change medium every three days till around 2 week. At this time we can see that there are some colonies formation but nearly no blood cells.
When the colonies are big enough, trypsinized and expand the cells. When the cells are enough, we can do FACS analysis and detect different markers.
Good luck and hopefully it can help you!
Best,
Shan
2018-12-04 09:04:09