José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Adison Wong Singapore Institute of Technology
1 protocol

Ali Kermani St Jude Children's Research Hospital
8 protocols

Alvaro Galvis University of Nevada Las Vegas
3 protocols

Anna Köhler Georg-August-Universität Göttingen
2 protocols

Editor
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics molecular biology microorganisms clinical pathogens bacteria yeast
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid, 2016

Lab information

Biotecnología microbiana UFV
José Berenguer's lab: Biotechnology of thermophilic microorganisms CBMSO
http://www.ufv.es/biotecnologia-microbiana http://web4.cbm.uam.es/joomla-rl/index.php/en/scientific-departments/virology-and-microbiology?id=%20718

Publications

2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 8608, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 8330, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
5 Protocols reviewed
Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 3014, Q&A: 0
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. ...
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Induction of Natural Competence in Genetically-modified Lactococcus lactis
Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 5648, Q&A: 0
Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence ...
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30 Protocols edited
Bacterial Growth Curve Measurements with a Multimode Microplate Reader
Authors:  Ariel T. Rogers, Kaitlin R. Bullard, Akash C. Dod and Yong Wang, date: 05/05/2022, view: 601, Q&A: 0

Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution,

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Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host
Authors:  Kim Shortall, Edmond Magner and Tewfik Soulimane, date: 05/05/2022, view: 514, Q&A: 0

Based on previous in-depth characterisation, aldehyde dehydrogenases (ALDH) are a diverse superfamily of enzymes, in terms of both structure and function, present in all kingdoms of life. They catalyse the oxidation of an aldehyde to carboxylic acid

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