Associate professor in Genetic Strains Research Center, National Institute of Genetics, and School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Japan.
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Kawasaki, T., Siegfried, K. R. and Sakai, N. (2016). Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Development 143(4): 566-574.
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Higaki, S., Shimada, M., Koyama, Y., Fujioka, Y., Sakai, N. and Takada, T. (2015). Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883). In Vitro Cell Dev Biol Anim 51(8): 763-768.
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Saito, K., Sakai, C., Kawasaki, T. and Sakai, N. (2014). Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Dev Dyn 243(11): 1448-1456.
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Nishimura, T., Herpin, A., Kimura, T., Hara, I., Kawasaki, T., Nakamura, S., Yamamoto, Y., Saito, T. L., Yoshimura, J., Morishita, S., Tsukahara, T., Kobayashi, S., Naruse, K., Shigenobu, S., Sakai, N., Schartl, M. and Tanaka, M. (2014). Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation. Development 141(17): 3363-3369.
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Shinya, M., Kobayashi, K., Masuda, A., Tokumoto, M., Ozaki, Y., Saito, K., Kawasaki, T., Sado, Y. and Sakai, N. (2013). Properties of gene knockdown system by vector-based siRNA in zebrafish. Dev Growth Differ 55(9): 755-765.
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Higaki, S., Koyama, Y., Shirai, E., Yokota, T., Fujioka, Y., Sakai, N. and Takada, T. (2013). Establishment of testicular and ovarian cell lines from Honmoroko (Gnathopogon caerulescens). Fish Physiol Biochem 39(3): 701-711.
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Higaki, S., Koyama, Y., Shimada, M., Ono, Y., Tooyama, I., Fujioka, Y., Sakai, N., Ikeuchi, T. and Takada, T. (2013). Response to fish specific reproductive hormones and endocrine disrupting chemicals of a Sertoli cell line expressing endogenous receptors from an endemic cyprinid Gnathopogon caerulescens. Gen Comp Endocrinol 191: 65-73.
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Kawasaki, T., Saito, K., Sakai, C., Shinya, M. and Sakai, N. (2012). Production of zebrafish offspring from cultured spermatogonial stem cells. Genes Cells 17(4): 316-325.
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Shinya, M. and Sakai, N. (2011). Generation of highly homogeneous strains of zebrafish through full sib-pair mating. G3 (Bethesda) 1(5): 377-386.
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Saito, K., Siegfried, K. R., Nusslein-Volhard, C. and Sakai, N. (2011). Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants. Dev Dyn 240(7): 1779-1792.
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Ozaki, Y., Saito, K., Shinya, M., Kawasaki, T. and Sakai, N. (2011). Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. Gene Expr Patterns 11(5-6): 309-315.
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Torihara, H., Uechi, T., Chakraborty, A., Shinya, M., Sakai, N. and Kenmochi, N. (2011). Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia. Br J Haematol 152(5): 648-654.
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Kawasaki, T., Saito, K., Shinya, M., Olsen, L. C. and Sakai, N. (2010). Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in Zebrafish (Danio rerio). Biol Reprod 83(4): 533-539.
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Kawasaki, T., Saito, K., Mitsui, K., Ikawa, M., Yamashita, M., Taniguchi, Y., Takeda, S., Mitani, K. and Sakai, N. (2009). Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. Zebrafish 6(3): 253-258.
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Hashiguchi, M., Shinya, M., Tokumoto, M. and Sakai, N. (2008). Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines. Dev Biol 321(2): 387-396.
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Kimura, T., Shimada, A., Sakai, N., Mitani, H., Naruse, K., Takeda, H., Inoko, H., Tamiya, G. and Shinya, M. (2007). Genetic analysis of craniofacial traits in the medaka. Genetics 177(4): 2379-2388.
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Sakai, N. (2006). In vitro male germ cell cultures of zebrafish. Methods 39(3): 239-245.