Corinne Mercier Université Grenoble Alpes, France
1 protocol

Jean Gagnon Labroatoire Adaptation et Pathogénie des Microorganismes (LAPM), France
2 protocols

Marie-France Cesbron-Delauw Université Grenoble Alpes, France
2 protocols

Gregory Effantin Université Grenoble Alpes, France
1 protocol

Nicolas Blanchard
  • Centre de Physiopathologie de Toulouse-Purpan (CPTP), France
Research focus
  • Immunology
  • 1 Author merit


Ph.D. in immunology and cell biology, Université Paris 5 René Descartes & Institut Curie, Paris, 2004

Current position

Team leader at Center for Pathophysiology of Toulouse-Purpan (CPTP) - INSERM UMR1043 - CNRS UMR5282 - University of Toulouse, France

Publications (since 2005)

  1. Sanecka, A., Yoshida, N., Dougan, S. K., Jackson, J., Shastri, N., Ploegh, H., Blanchard, N. and Frickel, E. M. (2016). Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden. Immunology.

  2. Chu, H. H., Chan, S. W., Gosling, J. P., Blanchard, N., Tsitsiklis, A., Lythe, G., Shastri, N., Molina-Paris, C. and Robey, E. A. (2016). Continuous Effector CD8(+) T Cell Production in a Controlled Persistent Infection Is Sustained by a Proliferative Intermediate Population. Immunity 45(1): 159-171.

  3. Lopez, J., Bittame, A., Massera, C., Vasseur, V., Effantin, G., Valat, A., Buaillon, C., Allart, S., Fox, B. A., Rommereim, L. M., Bzik, D. J., Schoehn, G., Weissenhorn, W., Dubremetz, J. F., Gagnon, J., Mercier, C., Cesbron-Delauw, M. F. and Blanchard, N. (2015). Intravacuolar Membranes Regulate CD8 T Cell Recognition of Membrane-Bound Toxoplasma gondii Protective Antigen. Cell Rep 13(10): 2273-2286.

  4. Wurm, R., Just, S., Wang, X., Wex, K., Schmid, U., Blanchard, N., Waisman, A., Schild, H. J., Deckert, M., Naumann, M., Schluter, D. and Nishanth, G. (2015). Protective dendritic cell responses against listeriosis induced by the short form of the deubiquitinating enzyme CYLD are inhibited by full-length CYLD. Eur J Immunol 45(5): 1366-1376.

  5. Joulia, R., Gaudenzio, N., Rodrigues, M., Lopez, J., Blanchard, N., Valitutti, S. and Espinosa, E. (2015). Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence. Nat Commun 6: 6174.

  6. Blanchard, N., Dunay, I. R. and Schluter, D. (2015). Persistence of Toxoplasma gondii in the central nervous system: a fine-tuned balance between the parasite, the brain and the immune system. Parasite Immunol 37(3): 150-158.

  7. Grover, H. S., Chu, H. H., Kelly, F. D., Yang, S. J., Reese, M. L., Blanchard, N., Gonzalez, F., Chan, S. W., Boothroyd, J. C., Shastri, N. and Robey, E. A. (2014). Impact of regulated secretion on antiparasitic CD8 T cell responses. Cell Rep 7(5): 1716-1728.

  8. Feliu, V., Vasseur, V., Grover, H. S., Chu, H. H., Brown, M. J., Wang, J., Boyle, J. P., Robey, E. A., Shastri, N. and Blanchard, N. (2013). Location of the CD8 T cell epitope within the antigenic precursor determines immunogenicity and protection against the Toxoplasma gondii parasite. PLoS Pathog 9(6): e1003449.

  9. Grover, H. S., Blanchard, N., Gonzalez, F., Chan, S., Robey, E. A. and Shastri, N. (2012). The Toxoplasma gondii peptide AS15 elicits CD4 T cells that can control parasite burden. Infect Immun 80(9): 3279-3288.

  10. Cebrian, I., Visentin, G., Blanchard, N., Jouve, M., Bobard, A., Moita, C., Enninga, J., Moita, L. F., Amigorena, S. and Savina, A. (2011). Sec22b regulates phagosomal maturation and antigen crosspresentation by dendritic cells. Cell 147(6): 1355-1368.

  11. Blanchard, N. and Shastri, N. (2010). Topological journey of parasite-derived antigens for presentation by MHC class I molecules. Trends Immunol 31(11): 414-421.

  12. Blanchard, N., Kanaseki, T., Escobar, H., Delebecque, F., Nagarajan, N. A., Reyes-Vargas, E., Crockett, D. K., Raulet, D. H., Delgado, J. C. and Shastri, N. (2010). Endoplasmic reticulum aminopeptidase associated with antigen processing defines the composition and structure of MHC class I peptide repertoire in normal and virus-infected cells. J Immunol 184(6): 3033-3042.

  13. Blanchard, N. and Shastri, N. (2010). Cross-presentation of peptides from intracellular pathogens by MHC class I molecules. Ann N Y Acad Sci 1183: 237-250.

  14. Blanchard, N., Gonzalez, F., Schaeffer, M., Joncker, N. T., Cheng, T., Shastri, A. J., Robey, E. A. and Shastri, N. (2008). Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum. Nat Immunol 9(8): 937-944.

  15. Blanchard, N. and Shastri, N. (2008). Coping with loss of perfection in the MHC class I peptide repertoire. Curr Opin Immunol 20(1): 82-88.

  16. Kanaseki, T., Blanchard, N., Hammer, G. E., Gonzalez, F. and Shastri, N. (2006). ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum. Immunity 25(5): 795-806.

  17. Miro, F., Nobile, C., Blanchard, N., Lind, M., Filipe-Santos, O., Fieschi, C., Chapgier, A., Vogt, G., de Beaucoudrey, L., Kumararatne, D. S., Le Deist, F., Casanova, J. L., Amigorena, S. and Hivroz, C. (2006). T cell-dependent activation of dendritic cells requires IL-12 and IFN-gamma signaling in T cells. J Immunol 177(6): 3625-3634.

  18. Bourbie-Vaudaine, S., Blanchard, N., Hivroz, C. and Romeo, P. H. (2006). Dendritic cells can turn CD4+ T lymphocytes into vascular endothelial growth factor-carrying cells by intercellular neuropilin-1 transfer. J Immunol 177(3): 1460-1469.

  19. Ballerini, C., Gourdain, P., Bachy, V., Blanchard, N., Levavasseur, E., Gregoire, S., Fontes, P., Aucouturier, P., Hivroz, C. and Carnaud, C. (2006). Functional implication of cellular prion protein in antigen-driven interactions between T cells and dendritic cells. J Immunol 176(12): 7254-7262.

1 Protocol published
Lipid Extraction from HeLa Cells, Quantification of Lipids, Formation of Large Unilamellar Vesicles (LUVs) by Extrusion and in vitro Protein-lipid Binding Assays, Analysis of the Incubation Product by Transmission Electron Microscopy (TEM) and by Flotation across a Discontinuous Sucrose Gradient
Dissecting the interactions established between proteins and membranes in a given type of cells is not an easy task. Using a cell-free system of large unilamellar vesicles (LUVs) to analyze these interactions may help decipher these interactions and ...
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