Yuya Katsuragi Graduate School of Biosciences, Nagahama Institute of Bio-Science and Technology, Japan
1 protocol

Takehito Furukawa Graduate School of Biosciences, Nagahama Institute of Bio-Science and Technology, Japan
1 protocol

Hiroyuki Hirai Nagahama Institute of Bio-Science and Technology
1 protocol

Fang-Sik Che
  • Graduate School of Biosciences, Nagahama Institute of Bio-Science and Technology, Japan
Research focus
  • Biochemistry
  • 1 Author merit


Ph. D in Agricultural Biochemistry, Department of Agriculture, University of Tokyo, 1991

Current position

Professor and chair in Graduate School of Biosciences, Nagahama Institute of Bio-Science and Technology, Nagahama, Japan

Publications (since 2005)

  1. Ootsubo, Y., Hibino, T., Wakazono, T., Mukai, Y. and Che, F. S. (2016). IREN, a novel EF-hand motif-containing nuclease, functions in the degradation of nuclear DNA during the hypersensitive response cell death in rice. Biosci Biotechnol Biochem 80(4): 748-760.

  2. Katsuragi, Y., Takai, R., Furukawa, T., Hirai, H., Morimoto, T., Katayama, T., Murakami, T. and Che, F. S. (2015). CD2-1, the C-Terminal Region of Flagellin, Modulates the Induction of Immune Responses in Rice. Mol Plant Microbe Interact 28(6): 648-658.

  3. Kamimura, M., Han, Y., Kito, N. and Che, F. S. (2014). Identification of interacting proteins for calcium-dependent protein kinase 8 by a novel screening system based on bimolecular fluorescence complementation. Biosci Biotechnol Biochem 78(3): 438-447.

  4. Furukawa, T., Inagaki, H., Takai, R., Hirai, H. and Che, F. S. (2014). Two distinct EF-Tu epitopes induce immune responses in rice and Arabidopsis. Mol Plant Microbe Interact 27(2): 113-124.

  5. Hirai, H., Takai, R., Kondo, M., Furukawa, T., Hishiki, T., Takayama, S. and Che, F. S. (2014). Glycan moiety of flagellin in Acidovorax avenae K1 prevents the recognition by rice that causes the induction of immune responses. Plant Signal Behav 9(11): e972782.

  6. Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Hasegawa, M., Komiya, T., Kondo, M., Che, F. S., Hanai, S. and Miwa, M. (2011). Approaches to separate and identify polyADP-ribosylated proteins using poly(ADP-ribose) glycohydrolase-knockout Drosophila. Methods Mol Biol 780: 377-390.

  7. Kondo, M., Yoshida, Y., Miyata, C., Fujiwara, S., Nakajima, Y., Hirai, H., Takayama, S., Isogai, A. and Che, F. S. (2012). Genetic organization of the hrp gene cluster in Acidovorax avenae strain N1141 and a novel effector protein that elicits immune responses in rice (Oryza sativa L.). Biosci Biotechnol Biochem 76(1): 129-138.

  8. Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A. and Che, F. S. (2011). Glycosylation regulates specific induction of rice immune responses by Acidovorax avenae flagellin. J Biol Chem 286(29): 25519-25530.

  9. Shinya, T., Osada, T., Desaki, Y., Hatamoto, M., Yamanaka, Y., Hirano, H., Takai, R., Che, F. S., Kaku, H. and Shibuya, N. (2010). Characterization of receptor proteins using affinity cross-linking with biotinylated ligands. Plant Cell Physiol, 51: 262-270

  10. Kakizaki, T., Matsumura, H., Nakayama, K., Che, F. S., Terauchi, R. and Inaba, T. (2009). Coordination of plastid protein import and nuclear gene expression by plastid-to-nucleus retrograde signaling. Plant Physiol 151(3): 1339-1353.

  11. Taga, Y., Takai, R., Kaneda, T., Matsui, H., Isogai, A. and Che, F. S. (2009). Role of OsHSP90 and IREN, Ca2+ dependent nuclease, in plant hypersensitive cell death induced by transcription factor OsNAC4. Plant Signal Behav 4(8): 740-742.

  12. Uchiyama, R., Aoki, K., Sugimoto, H., Taka, N., Katayama, T., Itonori, S., Sugita, M., Che, F. S., Kumagai, H. and Yamamoto, K. (2009). Phosphocholine-containing glycosyl inositol-phosphoceramides from Trichoderma viride induce defense responses in cultured rice cells. Biosci Biotechnol Biochem 73(1): 74-78.

  13. Kaneda, T., Taga, Y., Takai, R., Iwano, M., Matsui, H., Takayama, S., Isogai, A. and Che, F. S. (2009). The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death. EMBO J 28(7): 926-936.

  14. Takai, R., Isogai, A., Takayama, S. and Che, F. S. (2008). Analysis of flagellin perception mediated by flg22 receptor OsFLS2 in rice. Mol Plant Microbe Interact 21(12): 1635-1642.

  15. Takakura, Y., Che, F. S., Ishida, Y., Tsutsumi, F., Kurotani, K., Usami, S., Isogai, A. and Imaseki, H. (2008). Expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants. Mol Plant Pathol 9(4): 525-529.

  16. Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F. S., Watanabe, M., Isogai, A. and Takayama, S. (2007). Characterization of the SP11/SCR high-affinity binding site involved in self/nonself recognition in brassica self-incompatibility. Plant Cell 19(1): 107-117.

  17. Kaneda, T., Fujiwara, S., Takai, R., Takayama, S., Isogai, A. and Che, F. S. (2007). Identification of genes involved in induction of plant hypersensitive cell death. Plant Biotech. 24: 191-200.

  18. Takai, R., Kaneda, T., Isogai, A., Takayama, S. and Che, F. S. (2007). A new method of defense response analysis using a transient expression system in rice protoplasts. Biosci Biotechnol Biochem 71(2): 590-593.

  19. Tsujita, T., Ishii, A., Tsukada, H., Matsumoto, M., Che, F. S. and Seya, T. (2006). Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin. Vaccine 24(12): 2193-2199.

  20. Yoshie, Y., Goto, K., Takai, R., Iwano, M., Takayama, S., Isogai, S. and Che, F. S. (2005). Function of the rice gp91phox homologs OsrbohA and OsrbohE genes in ROS-dependent plant immune responses. Plant Biotech, 22: 127-135.

1 Protocol published
Purification of Flagellin from Acidovorax avenae and Analysis of Plant Immune Responses Induced by the Purified Flagellin
Authors:  Hiroyuki Hirai, Takehito Furukawa, Yuya Katsuragi and Fang-Sik Che, date: 08/20/2016, view: 7978, Q&A: 1
Plants sense potential pathogens by recognizing conserved pathogen-associated molecular patterns (PAMPs) that cause PAMP-triggered immunity (PTI) including the generation of reactive oxygen species, callose deposition, and expression of several ...
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