Dear Dr. Gillotin,
I tried this protocol for the separation of chromatin bound protein, but the isolation was not happened properly. Histone H3 was not detected in the chromatin part. Also there was tubulin contamination in the neuclear fraction. I have tried with both sonication based as well as be Benzonase based method and checked via Western Blot Analysis. Could you help me to by giving suggestions for these issues?
6/20/2022 12:34:44 AM Reply