François Guillemot The Francis Crick Institute, Mill Hill Laboratory, UK
1 protocol
Anthony Flamier Stem Cell and Developmental Biology Laboratory
6 protocols

David Delotterie Charles River Laboratories
4 protocols

Edgar Soria-Gomez Faculty of Medicine and Nursery, University of the Basque Country
27 protocols

Ehsan Kheradpezhouh Australian National University
20 protocols

Reviewer
Sébastien Gillotin
  • Research scientist, MSD, Discovery Research MRL
Research focus
  • Neuroscience
  • Neurogenesis, Transcription, Stem Cells, Aging, Metabolism
  • 2 Author merit

Education

Ph.D., Imperial College, London, 2007

Lab information

https://www.msd-uk.com/research/discovery-centre.xhtml

Publications

https://www.researchgate.net/profile/Sebastien_Gillotin
  1. Gillotin, S., Davies, J. D. and Philpott, A. (2018). Subcellular localisation modulates ubiquitylation and degradation of Ascl1.Sci Rep 8(1): 4625.  

  2. Gillotin, S. and Guillemot, F. (2016). Micro-chromatin Immunoprecipation(muChIP)Protocol for Real-time PCR Analysis of a Limited Amount of Cells.Bio Protoc 6(12). 

  3. Raposo, A. A., Vasconcelos, F. F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., Bithell, A., Gillotin, S., vanden Berg, D. L., Ettwiller, L., Flicek, P., Crawford, G. E., Parras, C. M., Berninger, B., Buckley, N. J., Guillemot, F. and Castro, D. S. (2015).Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep

  4. Masserdotti, G., Gillotin, S., Sutor, B., Drechsel, D., Irmler, M., Jorgensen, H. F., Sass, S., Theis, F. J., Beckers, J., Berninger, B., Guillemot, F. and Gotz, M. (2015).Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes.Cell Stem Cell 17(1): 74-88. 

  5. Gillotin, S. and Lu, X. (2011).The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.FEBS Lett585(12): 1778-1782. 

  6. Gillotin, S., Yap, D. and Lu, X. (2010).Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation.Cell Cycle 9(7): 1390-1398. 

  7. Gillotin, S. (2009). iASPP,a potential drug target in cancer therapy.Leuk Res 33(9): 1175-1177. 

  8. Slee, E. A., Gillotin, S., Bergamaschi, D., Royer, C., Llanos, S., Ali, S., Jin, B., Trigiante, G. and Lu, X. (2004). The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.Oncogene 23(56): 9007-9016.

2 Protocols published
Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Author:  Sébastien Gillotin, date: 10/05/2018, view: 16059, Q&A: 6
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often ...
Micro-chromatin Immunoprecipitation (μChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Authors:  Sébastien Gillotin and François Guillemot, date: 06/20/2016, view: 8211, Q&A: 0
Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is an important strategy to study gene regulation. When availability of cells is limited, however, it can be useful to focus on specific genes to investigate in depth the role of ...
12 Protocols reviewed
Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia
Authors:  Joanne L. Sharpe, Nikki S. Harper and Ryan J. H. West, date: 05/20/2022, view: 577, Q&A: 0

Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide

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A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
Authors:  Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale, date: 06/20/2021, view: 2030, Q&A: 0

Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the

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