Ming Luo Chinese Academy of Sciences
1 protocol

Keqiang Wu
  • Institute of Plant Biology, College of Life Science, National Taiwan University, Taiwan
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Molecular Biology, University of Saskatchewan, Canada, 1994

Current position

Professor, Institute of Plant Biology, National Taiwan University, Taiwan


Peer-reviewed Journals (*Corresponding author)

  1. Chen, C. Y., Wu, K. and Schmidt, W. (2015). The histone deacetylase HDA19 controls root cell elongation and modulates a subset of phosphate starvation responses in Arabidopsis. Sci Rep 5: 15708.
  2. Yang, S., Li, C., Zhao, L., Gao, S., Lu, J., Zhao, M., Chen, C. Y., Liu, X., Luo, M., Cui, Y., Yang, C. and Wu, K.* (2015). The Arabidopsis SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA Targets Directly to PINs and Is Required for Root Stem Cell Niche Maintenance. Plant Cell 27(6): 1670-1680.
  3. Luo, M., Tai, R., Yu, C. W., Yang, S., Chen, C. Y., Lin, W. D., Schmidt, W. and Wu, K.* (2015). Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis. Plant J 82(6): 925-936.
  4. Zhao, M., Yang, S., Chen, C. Y., Li, C., Shan, W., Lu, W., Cui, Y., Liu, X. and Wu, K.* (2015). Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture. PLoS Genet 11(3): e1005125.
  5. Zhao, M., Yang, S., Liu, X. and Wu, K. (2015). Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes. Front Plant Sci 6: 159.
  6. Li, C., Chen, C., Gao, L., Yang, S., Nguyen, V., Shi, X., Siminovitch, K., Kohalmi, S. E., Huang, S., Wu, K., Chen, X. and Cui, Y. (2015). The Arabidopsis SWI2/SNF2 chromatin Remodeler BRAHMA regulates polycomb function during vegetative development and directly activates the flowering repressor gene SVP. PLoS Genet 11(1): e1004944.
  7. Zhao, L., Lu, J., Zhang, J., Wu, P. Y., Yang, S. and Wu, K.* (2014). Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum). Front Plant Sci 5: 760.
  8. Hsu, C. C., Wu, P. S., Chen, T. C., Yu, C. W., Tsai, W. C., Wu, K., Wu, W. L., Chen, W. H. and Chen, H. H. (2014). Histone acetylation accompanied with promoter sequences displaying differential expression profiles of B-class MADS-box genes for phalaenopsis floral morphogenesis. PLoS One 9(12): e106033.
  9. Singh, P., Yekondi, S., Chen, P. W., Tsai, C. H., Yu, C. W., Wu, K. and Zimmerli, L. (2014). Environmental History Modulates Arabidopsis Pattern-Triggered Immunity in a HISTONE ACETYLTRANSFERASE1-Dependent Manner. Plant Cell 26(6): 2676-2688.
  10. Liu, X., Yang, S., Zhao, M., Luo, M., Yu, C. W., Chen, C. Y., Tai, R. and Wu, K.* (2014). Transcriptional repression by histone deacetylases in plants. Mol Plant 7(5): 764-772.
  11. Luo M, Hung F, Yang S, Liu X, and Wu K* (2014) Histone lysine demethylases and their functions in plants. Plant Mol Biol Rep 32:558–565.
  12. Yuan, L., Liu, X., Luo, M., Yang, S. and Wu, K.* (2013). Involvement of histone modifications in plant abiotic stress responses. J Integr Plant Biol 55(10): 892-901.
  13. Liu, X., Chen, C. Y., Wang, K. C., Luo, M., Tai, R., Yuan, L., Zhao, M., Yang, S., Tian, G., Cui, Y., Hsieh, H. L. and Wu, K.* (2013). PHYTOCHROME INTERACTING FACTOR3 associates with the histone deacetylase HDA15 in repression of chlorophyll biosynthesis and photosynthesis in etiolated Arabidopsis seedlings. Plant Cell 25(4): 1258-1273.
  14. Zhou, Y., Tan, B., Luo, M., Li, Y., Liu, C., Chen, C., Yu, C. W., Yang, S., Dong, S., Ruan, J., Yuan, L., Zhang, Z., Zhao, L., Li, C., Chen, H., Cui, Y., Wu, K.* and Huang, S.* (2013). HISTONE DEACETYLASE19 interacts with HSL1 and participates in the repression of seed maturation genes in Arabidopsis seedlings. Plant Cell 25(1): 134-148.
  15. Luo, M., Yu, C. W., Chen, F. F., Zhao, L., Tian, G., Liu, X., Cui, Y., Yang, J. Y. and Wu, K.* (2012). Histone deacetylase HDA6 is functionally associated with AS1 in repression of KNOX genes in arabidopsis. PLoS Genet 8(12): e1003114.
  16. Luo, M., Wang, Y. Y., Liu, X., Yang, S., Lu, Q., Cui, Y. and Wu, K.* (2012). HD2C interacts with HDA6 and is involved in ABA and salt stress response in Arabidopsis. J Exp Bot 63(8): 3297-3306.
  17. Alinsug, M. V., Chen, F. F., Luo, M., Tai, R., Jiang, L. and Wu, K.* (2012). Subcellular localization of class II HDAs in Arabidopsis thaliana: nucleocytoplasmic shuttling of HDA15 is driven by light. PLoS One 7(2): e30846.
  18. Liu, X., Yu, C. W., Duan, J., Luo, M., Wang, K., Tian, G., Cui, Y. and Wu, K.* (2012). HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable element silencing in Arabidopsis. Plant Physiol 158(1): 119-129.
  19. Liu, X., Luo, M., Zhang, W., Zhao, J., Zhang, J., Wu, K.*, Tian, L.* and Duan, J.* (2012). Histone acetyltransferases in rice (Oryza sativa L.): phylogenetic analysis, subcellular localization and expression. BMC Plant Biol 12: 145.
  20. Gao, Y., Yang, S., Yuan, L., Cui, Y. and Wu, K.* (2012). Comparative Analysis of SWIRM Domain-Containing Proteins in Plants. Comp Funct Genomics 2012: 310402.
  21. Luo, M., Wang, Y. Y., Liu, X., Yang, S. and Wu, K.* (2012). HD2 proteins interact with RPD3-type histone deacetylases. Plant Signal Behav 7(6): 608-610.
  22. Liu, X., Luo, M. and Wu, K.* (2012). Epigenetic interplay of histone modifications and DNA methylation mediated by HDA6. Plant Signal Behav 7(6): 633-635.
  23. Luo, M., Liu, X., Singh, P., Cui, Y., Zimmerli, L. and Wu, K.* (2012). Chromatin modifications and remodeling in plant abiotic stress responses. Biochim Biophys Acta 1819(2): 129-136.
  24. Zhou, Y., Chu, P., Chen, H., Li, Y., Liu, J., Ding, Y., Tsang, E. W., Jiang, L., Wu, K. and Huang, S. (2012). Overexpression of Nelumbo nucifera metallothioneins 2a and 3 enhances seed germination vigor in Arabidopsis. Planta 235(3): 523-537.
  25. Yu, C. W., Liu, X., Luo, M., Chen, C., Lin, X., Tian, G., Lu, Q., Cui, Y. and Wu, K.* (2011). HISTONE DEACETYLASE6 interacts with FLOWERING LOCUS D and regulates flowering in Arabidopsis. Plant Physiol 156(1): 173-184.
  26. Chen, L. T. and Wu, K.* (2010). Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response. Plant Signal Behav 5(10): 1318-1320.
  27. Chen, L. T., Luo, M., Wang, Y. Y. and Wu, K.* (2010). Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response. J Exp Bot 61(12): 3345-3353.
  28. Song, Y., Wu, K., Dhaubhadel, S., An, L. and Tian, L. (2010). Arabidopsis DNA methyltransferase AtDNMT2 associates with histone deacetylase AtHD2s activity. Biochem Biophys Res Commun 396(2): 187-192.
  29. Fu, G., Zhong, Y., Li, C., Li, Y., Lin, X., Liao, B., Tsang, E. W., Wu, K.* and Huang, S. (2010). Epigenetic regulation of peanut allergen gene Ara h 3 in developing embryos. Planta 231(5): 1049-1060.
  30. Li, C., Wu, K., Fu, G., Li, Y., Zhong, Y., Lin, X., Zhou, Y., Tian, L. and Huang, S. (2009). Regulation of oleosin expression in developing peanut (Arachis hypogaea L.) embryos through nucleosome loss and histone modifications. J Exp Bot 60(15): 4371-4382.
  31. Alinsug, M. V., Yu, C. W. and Wu, K.* (2009). Phylogenetic analysis, subcellular localization, and expression patterns of RPD3/HDA1 family histone deacetylases in plants. BMC Plant Biol 9: 37.
  32. Wu, K.*, Zhang, L., Zhou, C., Yu, C. W. and Chaikam, V. (2008). HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J Exp Bot 59(2): 225-234.
  33. Liu, Z., Zhou, C. and Wu, K.* (2008). Creation and analysis of a novel chimeric promoter for the complete containment of pollen- and seed-mediated gene flow. Plant Cell Rep 27(6): 995-1004.
  34. Chiou, C. Y., Wu, K. and Yeh, K. W. (2008). Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey. Biotechnol Lett 30(10): 1861-1866.
  35. Fu, W., Wu, K.* and Duan, J. (2007). Sequence and expression analysis of histone deacetylases in rice. Biochem Biophys Res Commun 356(4): 843-850.
  36. Sridha, S. and Wu, K.* (2006). Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis. Plant J 46(1): 124-133.
  37. Zhou, C., Zhang, L., Duan, J., Miki, B. and Wu, K.* (2005). HISTONE DEACETYLASE19 is involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis. Plant Cell 17(4): 1196-1204.
  38. Yang, Z., Tian, L., Latoszek-Green, M., Brown, D. and Wu, K.* (2005). Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. Plant Mol Biol 58(4): 585-596.
  39. Yan Y., Lin X., Zhang Y., Wang L., Li Y., Wu K. and Huang S (2005) Isolation of peanut cDNAs encoding arachins and conglutins by expressed sequence tags. Plant Sci. 169: 439-445.
  40. Tian, L., Wu, K., Hannam, C., Latoszek-Green, M., Sibbald, S., Hu, M., Brown, D. C. and Miki, B. (2005). Analysis and use of the tobacco eIF4A-10 promoter elements for transgene expression. J Plant Physiol 162(12): 1355-1366.
  41. Zhou, C., Labbe, H., Sridha, S., Wang, L., Tian, L., Latoszek-Green, M., Yang, Z., Brown, D., Miki, B. and Wu, K.* (2004). Expression and function of HD2-type histone deacetylases in Arabidopsis development. Plant J 38(5): 715-724.
  42. Zhou, C., Miki, B. and Wu, K.* (2003). CHB2, a member of the SWI3 gene family, is a global regulator in Arabidopsis. Plant Mol Biol 52(6): 1125-1134.
  43. Wu, K.*, Tian, L., Zhou, C., Brown, D. and Miki, B. (2003). Repression of gene expression by Arabidopsis HD2 histone deacetylases. Plant J 34(2): 241-247.
  44. Wu, K., Hu, M., Martin, T., Wang, C., Li, X. Q., Tian, L., Brown, D. and Miki, B. (2003). The cryptic enhancer elements of the tCUP promoter. Plant Mol Biol 51(3): 351-362.
  45. Tian L., Wu K., Ouellet T., Foster E., Latoszek-Green M., Sibbald S., Miki B., Seguin and Brown D. (2003) Activity of elements from the tobacco cryptic promoter, tcup, in conifer tissues. In Vitro Cell Devel Biol - Plant 39:193-202.
  46. Wu, K., Tian, L., Hollingworth, J., Brown, D. C. and Miki, B. (2002). Functional analysis of tomato Pti4 in Arabidopsis. Plant Physiol 128(1): 30-37.
  47. Miki B., Brown D., Tian L., Malik K., and Wu K. (2002) The role of histone deacetylases in flowering. Flowering Newsletter 33: 3-6.
  48. Tian L., Wang, H., Wu K., Latoszek-Green M., Hu M., Miki B., and Brown D. (2002) Efficient recovery of transgenic plants through organogenesis and embryogenesis using a cryptic promoterto drive marker gene expression. Plant Cell Rep. 20:1181-1187.
  49. Malik, K., Wu, K., Li, X. Q., Martin-Heller, T., Hu, M., Foster, E., Tian, L., Wang, C., Ward, K., Jordan, M., Brown, D., Gleddie, S., Simmonds, D., Zheng, S., Simmonds, J. and Miki, B. (2002). A constitutive gene expression system derived from the tCUP cryptic promoter elements. Theor Appl Genet 105(4): 505-514.
  50. Akache, B., Wu, K. and Turcotte, B. (2001). Phenotypic analysis of genes encoding yeast zinc cluster proteins. Nucleic Acids Res 29(10): 2181-2190.
  51. Wu, K., Malik, K., Tian, L., Hu, M., Martin, T., Foster, E., Brown, D. and Miki, B. (2001). Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP. Mol Genet Genomics 265(5): 763-770.
  52. Wu, K., Tian, L., Malik, K., Brown, D. and Miki, B. (2000). Functional analysis of HD2 histone deacetylase homologues in Arabidopsis thaliana. Plant J 22(1): 19-27.
  53. Wu, K., Malik, K., Tian, L., Brown, D. and Miki, B. (2000). Functional analysis of a RPD3 histone deacetylase homologue in Arabidopsis thaliana. Plant Mol Biol 44(2): 167-176.
  54. Wu, K., Li, L., Gage, D. A. and Zeevaart, J. A. (1996). Molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach. Plant Physiol 110(2): 547-554.
  55. Xu, Y. L., Li, L., Wu, K., Peeters, A. J., Gage, D. A. and Zeevaart, J. A. (1995). The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. Proc Natl Acad Sci U S A 92(14): 6640-6644.
  56. Wu, K., Cossins, E. A. and King, J. (1994). Folate Metabolism in Datura innoxia (In Vivo and in Vitro Folylpolyglutamate Synthesis in Wild-Type and Methotrexate-Resistant Cells). Plant Physiol 104(2): 373-380.
  57. Wu K., Mourad G. and King J. (1994) A valine-resistant mutant of Arabidopsis displays an acetolactate synthase with changed feedback control. Planta 192: 249-255.
  58. Wu K. and King J. (1994) Biochemical and genetic characterization of 5-Fluoro-2'-deoxyuridine resistant mutants of Arabidopsis. Planta 197: 117-122.
  59. Wu K., Atkinson I., Cossins E. and King J. (1993) Methotrexate resistance in Datura innoxia. Uptake and metabolism of methotrexate in wild type and resistant cells. Plant Physiol. 101: 477-483.

Book chapters

  1. Liu, X., Luo, M., Yang, S. and Wu, K.* (2015). Role of Epigenetic Modifications in Plant Responses to Environmental Stresses. In: Pontes, O. and Jin, H. (eds). Nuclear Functions in Plant Transcription and Signaling.  Springer Science+Business Media, 81-92.
  2. Miki, B. and Wu, K. (2003). Control of gene expression by histone deacetylases. In: Vasil,I. K. (ed). Plant Biotechnology 2002 and Beyond. Kluwer Academic Publishers, 211-214.
  3. Zhang, C. Q., Liu, Y., Eschen, W. M. and Wu, K. (2003). Identifying regulatory signals in DNA-sequences with a non-statistical approximation approach. In: Proceeding of the 2003 IEEE Bioinformatics Conference. 593-597.

1 Protocol published
Expression, Purification and Enzymatic Assay of Plant Histone Deacetylases
Authors:  Ming Luo and Keqiang Wu, date: 04/05/2016, view: 8036, Q&A: 0
Histone deacetylases (HDACs) catalyzing the removal of acetyl groups from lysine residues of histone and non-histone proteins play vital roles in regulation of gene transcription. In plants, HDACs can be grouped into three families, including ...
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