Hello,I just followed ur protocal,but I met some problems,my crypt cells vacuolated in the first day,but they can't bud,then they apoptosis in the next few days.I don't know if there is a problem with the use of my medium,How do you save them and add cell growth factors?Please kindly help me to troubleshoot the problem.
7/19/2018 10:04:00 PM Reply