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Ming Lei
  • National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
Research fields
  • Biochemistry
Personal information

Education

Ph.D. in Biophysics, Laboratory of Molecular Medicine at Children’s Hospital Boston, Harvard University, Cambridge, MA, 2001

Current position

Director, National Center for Protein Science Shanghai, China (06/2011-present)
Deputy Director, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China (06/2011-present)
Associate Professor (tenured), Department of Biological Chemistry, University of Michigan Medical School (09/2010-present) 
Joint faculty, Biophysics Program, University of Michigan (09/2006-present) 
Joint faculty, Chemical Biology Program, University of Michigan (09/2007-present)   

Publications

Peer-reviewed journals

  1. Vallabhaneni, H.#, Zhou, F., Maul, R. W., Sarkar, J., Yin, J., Lei, M., Harrington, L., Gearhart, P. J.* and Liu, Y.* (2015). Defective repair of uracil causes telomere defects in mouse hematopoietic cells. J Biol Chem 290(9): 5502-5511.
  2. Sommers, J. A.#, Banerjee, T., Hinds, T., Wan, B., Wold, M. S., Lei, M. and Brosh, R. M., Jr.* (2014). Novel function of the Fanconi anemia group J or RECQ1 helicase to disrupt protein-DNA complexes in a replication protein A-stimulated manner. J Biol Chem 289(29): 19928-19941.
  3. Huang, J.#, Brown, A. F., Wu, J., Xue, J., Bley, C. J., Rand, D. P., Wu, L., Zhang, R., Chen, J. J.* and Lei, M.* (2014). Structural basis for protein-RNA recognition in telomerase. Nat Struct Mol Biol 21(6): 507-512.
  4. Yang, Y. W.#, Flynn, R. A., Chen, Y., Qu, K., Wan, B., Wang, K. C., Lei, M. and Chang, H. Y.* (2014). Essential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency. Elife 3: e02046.
  5. Cao, F.#, Townsend, E. C.#, Karatas, H.#, Xu, J., Li, L., Lee, S., Liu, L., Chen, Y., Ouillette, P., Zhu, J., Hess, J. L., Atadja, P., Lei, M., Qin, Z. S., Malek, S., Wang, S.* and Dou, Y.* (2014). Targeting MLL1 H3K4 methyltransferase activity in mixed-lineage leukemia. Mol Cell 53(2): 247-261.
  6. Zhou, H.#, Liu, L., Huang, J., Bernard, D., Karatas, H., Navarro, A., Lei, M. and Wang, S.* (2013). Structure-based design of high-affinity macrocyclic peptidomimetics to block the menin-mixed lineage leukemia 1 (MLL1) protein-protein interaction. J Med Chem 56(3): 1113-1123.
  7. Wan, B.#, Yin, J.#, Horvath, K.#, Sarkar, J., Chen, Y., Wu, J., Wan, K., Lu, J., Gu, P., Yu, E. Y., Lue, N. F., Chang, S., Liu, Y.* and Lei, M.* (2013). SLX4 assembles a telomere maintenance toolkit by bridging multiple endonucleases with telomeres. Cell Rep 4(5): 861-869.
  8. Karatas, H.#, Townsend, E. C.#, Cao, F., Chen, Y., Bernard, D., Liu, L., Lei, M., Dou, Y. and Wang, S.* (2013). High-affinity, small-molecule peptidomimetic inhibitors of MLL1/WDR5 protein-protein interaction. J Am Chem Soc 135(2): 669-682.
  9. Lue, N. F.*, Yu, E. Y. and Lei, M. (2013). A popular engagement at the ends. Nat Struct Mol Biol 20(1): 10-12.
  10. Gu, P.#, Deng, W., Lei, M. and Chang, S.* (2013). Single strand DNA binding proteins 1 and 2 protect newly replicated telomeres. Cell Res 23(5): 705-719.
  11. Yu, E. Y.#, Sun, J.#, Lei, M.* and Lue, N. F.* (2012). Analyses of Candida Cdc13 orthologues revealed a novel OB fold dimer arrangement, dimerization-assisted DNA binding, and substantial structural differences between Cdc13 and RPA70. Mol Cell Biol 32(1): 186-198.
  12. Thiel, A. T.#, Huang, J., Lei, M. and Hua, X.* (2012). Menin as a hub controlling mixed lineage leukemia. Bioessays 34(9): 771-780.
  13. Huang, J.#, Wan, B., Wu, L., Yang, Y., Dou, Y. and Lei, M.* (2012). Structural insight into the regulation of MOF in the male-specific lethal complex and the non-specific lethal complex. Cell Res 22(6): 1078-1081.
  14. Huang, J.#, Gurung, B., Wan, B., Matkar, S., Veniaminova, N. A., Wan, K., Merchant, J. L., Hua, X.* and Lei, M.* (2012). The same pocket in menin binds both MLL and JUND but has opposite effects on transcription. Nature 482(7386): 542-546.
  15. Chen, Y.#, Cao, F., Wan, B., Dou, Y. and Lei, M.* (2012). Structure of the SPRY domain of human Ash2L and its interactions with RbBP5 and DPY30. Cell Res 22(3): 598-602.
  16. Zeng, Z.#, Min, B.#, Huang, J., Hong, K., Yang, Y., Collins, K.* and Lei, M.* (2011). Structural basis for Tetrahymena telomerase processivity factor Teb1 binding to single-stranded telomeric-repeat DNA. Proc Natl Acad Sci U S A 108(51): 20357-20361.
  17. Yen, W. F.#, Chico, L., Lei, M. and Lue, N. F.* (2011). Telomerase regulatory subunit Est3 in two Candida species physically interacts with the TEN domain of TERT and telomeric DNA. Proc Natl Acad Sci U S A 108(51): 20370-20375.
  18. Wang, K. C.#, Yang, Y. W., Liu, B., Sanyal, A., Corces-Zimmerman, R., Chen, Y., Lajoie, B. R., Protacio, A., Flynn, R. A., Gupta, R. A., Wysocka, J., Lei, M., Dekker, J., Helms, J. A. and Chang, H. Y.* (2011). A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature 472(7341): 120-124.
  19. Sun, J.#, Yang, Y., Wan, K., Mao, N., Yu, T. Y., Lin, Y. C., DeZwaan, D. C., Freeman, B. C., Lin, J. J., Lue, N. F. and Lei, M.* (2011). Structural bases of dimerization of yeast telomere protein Cdc13 and its interaction with the catalytic subunit of DNA polymerase alpha. Cell Res 21(2): 258-274.
  20. Chen, Y.#, Wan, B.#, Wang, K. C.#, Cao, F., Yang, Y., Protacio, A., Dou, Y., Chang, H. Y. and Lei, M.* (2011). Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding. EMBO Rep 12(8): 797-803.
  21. Chen, Y.#, Rai, R.#, Zhou, Z. R., Kanoh, J., Ribeyre, C., Yang, Y., Zheng, H., Damay, P., Wang, F., Tsujii, H., Hiraoka, Y., Shore, D., Hu, H. Y., Chang, S.* and Lei, M.* (2011). A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms. Nat Struct Mol Biol 18(2): 213-221.
  22. Zeng, Z.#, Wang, W.#, Yang, Y., Chen, Y., Yang, X., Diehl, J. A., Liu, X.* and Lei, M.* (2010). Structural basis of selective ubiquitination of TRF1 by SCFFbx4. Dev Cell 18(2): 214-225.
  23. Wang, F.#, Yang, Y., Singh, T. R., Busygina, V., Guo, R., Wan, K., Wang, W., Sung, P., Meetei, A. R. and Lei, M.* (2010). Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex. Structure 18(9): 1159-1170.
  24. Cao, F.#, Chen, Y.#, Cierpicki, T., Liu, Y., Basrur, V., Lei, M.* and Dou, Y.* (2010). An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain. PLoS One 5(11): e14102.
  25. Wang, W.#, Yang, Y., Gao, Y., Xu, Q., Wang, F., Zhu, S., Old, W., Resing, K., Ahn, N., Lei, M.* and Liu, X.* (2009). Structural and mechanistic insights into Mps1 kinase activation. J Cell Mol Med 13(8B): 1679-1694.
  26. Sun, J.#, Yu, E. Y.#, Yang, Y., Confer, L. A., Sun, S. H., Wan, K., Lue, N. F.* and Lei, M.* (2009). Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres. Genes Dev 23(24): 2900-2914.
  27. Yu, E. Y.#, Wang, F., Lei, M. and Lue, N. F.* (2008). A proposed OB-fold with a protein-interaction surface in Candida albicans telomerase protein Est3. Nat Struct Mol Biol 15(9): 985-989.
  28. Sowd, G.#, Lei, M. and Opresko, P. L.* (2008). Mechanism and substrate specificity of telomeric protein POT1 stimulation of the Werner syndrome helicase. Nucleic Acids Res 36(13): 4242-4256.
  29. Chen, Y.#, Yang, Y., van Overbeek, M., Donigian, J. R., Baciu, P., de Lange, T. and Lei, M.* (2008). A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins. Science 319(5866): 1092-1096.
  30. Wang, F.#, Podell, E. R., Zaug, A. J., Yang, Y., Baciu, P., Cech, T. R. and Lei, M.* (2007). The POT1-TPP1 telomere complex is a telomerase processivity factor. Nature 445(7127): 506-510.
  31. Chen, Y.#, Yang, Y., Wang, F., Wan, K., Yamane, K., Zhang, Y. and Lei, M.* (2006). Crystal structure of human histone lysine-specific demethylase 1 (LSD1). Proc Natl Acad Sci U S A 103(38): 13956-13961.
  32. Opresko, P. L.#, Mason, P. A., Podell, E. R., Lei, M., Hickson, I. D., Cech, T. R. and Bohr, V. A.* (2005). POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates. J Biol Chem 280(37): 32069-32080.
  33. Lei, M.#, Zaug, A. J., Podell, E. R. and Cech, T. R.* (2005). Switching human telomerase on and off with hPOT1 protein in vitro. J Biol Chem 280(21): 20449-20456.
  34. Lei, M.#, Robinson, M. A. and Harrison, S. C.* (2005). The active conformation of the PAK1 kinase domain. Structure 13(5): 769-778.
  35. Lei, M.#, Podell, E. R. and Cech, T. R.* (2004). Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection. Nat Struct Mol Biol 11(12): 1223-1229.
  36. Lei, M.#, Podell, E. R., Baumann, P. and Cech, T. R.* (2003). DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA. Nature 426(6963): 198-203.
  37. Parrini, M. C.#, Lei, M., Harrison, S. C. and Mayer, B. J.* (2002). Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1. Mol Cell 9(1): 73-83.
  38. Lei, M.#, Baumann, P. and Cech, T. R.* (2002). Cooperative binding of single-stranded telomeric DNA by the Pot1 protein of Schizosaccharomyces pombe. Biochemistry 41(49): 14560-14568.
  39. Lowry, W. E.#, Huang, J., Lei, M., Rawlings, D. and Huang, X. Y.* (2001). Role of the PHTH module in protein substrate recognition by Bruton's agammaglobulinemia tyrosine kinase. J Biol Chem 276(48): 45276-45281.
  40. Lei, M.#, Lu, W., Meng, W., Parrini, M. C., Eck, M. J., Mayer, B. J. and Harrison, S. C.* (2000). Structure of PAK1 in an autoinhibited conformation reveals a multistage activation switch. Cell 102(3): 387-397.
  41. Xu, W.#, Doshi, A., Lei, M., Eck, M. J. and Harrison, S. C.* (1999). Crystal structures of c-Src reveal features of its autoinhibitory mechanism. Mol Cell 3(5): 629-638.
  42. Quadri, L. E.#, Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P. and Walsh, C. T.* (1998). Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases. Biochemistry 37(6): 1585-1595.
  43. Lei, M.# and Guo, H.* (1996). Numerical confirmation of universality of transmission microsymmetry relations in a four-probe quantum dot. Phys Rev B Condens Matter 54(16): 11630-11637.
  44. Zhu, N.#, Lei, M. and Guo, H.* (1996). Magnetoresistance oscillation in window-coupled wire systems. J Appl Phys 79(9): 7036-40.
  45.  Lei, M.#, Zhu, N. and Guo, H.* (1995). Quantum Hall effect in the presence of an antidot potential. Phys Rev B Condens Matter 52(23): 16784-16792.

Book chapters

  1. Wang, F. and Lei, M. (2010). Human telomere POT1-TPP1 complex and its role in telomerase activity regulation, in telomeres and telomerase: Methods and protocols. Second Edition.
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