Pedro Prudêncio Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Portugal
2 protocols
Jian Chen Washington University School of Medicine
9 protocols

Jihyun Kim Finless Foods
20 protocols

Jyotiska Chaudhuri Baxter Healthcare Corporation
28 protocols

Masahiro Morita University of Texas Health Science Center at San Antonio
29 protocols

Leonardo Gaston Guilgur
  • Post-Doc, Instituto Gulbenkian de Ciencia
Research focus
  • Developmental biology
  • Gene expression regulation, Aneuploidy, Drosophila development
  • 2 Author merit


PhD, Universidad Nacional de San Martin, Argentina, 2008


1.Navarro-Costa P., McCarthy A., Prudêncio, P., Greer C., Guilgur L.G., Becker J.D., Se- combe J., Rangan P., Martinho R.G. 2016. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nat Commun. 10; 7:12331. Impact factor: 11.329 SCR indicator: Biochemis- try, Genetics and Molecular Biology Q1 6,539

2. Prudêncio P., Guilgur L.G. 2015. Protein Extraction from Drosophila Embryos and Ova- ries. Bio-protocol 5(9): e1459.

3. Prudêncio P., Guilgur L.G. 2015. FLP/FRT Induction of Mitotic Recombination in Dro- sophila. Bio-protocol 5(9): e1458.

4. Martinho R.G., Guilgur L.G., Prudêncio P. 2015. How gene expression in fast-proliferating cells keeps pace. BioEssays in press Impact factor: 4.838 SCR indicator: Biochemistry, Genetics and Molecular Biology Q1 3,251

5. Guilgur L.G., Prudêncio P., Sobral D., Liszekova D., Rosa A., Martinho R.G. 2014. Requi- rement for highly efficient pre-mRNA splicing during Drosophila early embryonic deve- lopment. eLife. doi: 10.7554/eLife.02181 Impact Factor: 8,519 SCR indicator: Bioche- mistry, Genetics and Molecular Biology Q1 4,329

6. Guilgur L.G., Prudêncio P., Ferreira T., Pimenta-Marques A.R., Martinho R.G. 2012. Dro- sophila aPKC is required for mitotic spindle orientation during symmetric division of epit- helial cells. Development. 139: 503-13. Impact Factor: 6,7 SCR indicator: Developmental biology Q1 5,75. *Highlighted in Journal of Cell Science

7. Blasco M., Fernandino J.I., Guilgur L.G., Strüssmann C.A., Somoza G.M., Vizziano-Can- tonnet D. 2010. Molecular characterization of cyp11a1 and cyp11b1 and their gene expres- sion profile in pejerrey (Odontesthes bonariensis) during early gonadal development. Comp Biochem Physiol A Mol Integr Physiol. 156:110-8. Impact Factor: 2,371 SCR indicator: Physiology Q2 0,947

8. Guilgur L.G., Strüssmann C.A., Somoza G.M. 2009. mRNA expression of GnRH variants and receptors in the brain, pituitary and ovaries of pejerrey (Odontesthes bonariensis) in relation to the reproductive status. Fish Physiol. Biochem. 35: 157-66. Impact Factor: 1,676 SCR indicator: Physiology Q3 0,692

9. Guilgur L.G., Ortí G., Strobl-Mazzulla P.H., Fernandino J.I., Miranda L.A., Somoza G.M. 2007. Characterization of the cDNAs encoding three GnRH forms in the pejerrey fish Odontesthes bonariensis (Atheriniformes) and the evolution of GnRH precursors. Journal of Molecular Evolution. 64(6): 614-27. Impact Factor: 1,863 SCR indicator: Evolution Q1 1,070

10. Miranda L.A., Strüssmann C.A., Guilgur L.G., Strobl-Mazzulla P.H., Somoza G.M. 2007. Cloning of FSH-ß, LH-ß and glicoprotein hormone α subunits in pejerrey (Odontesthes bo- nariensis, Atheriniformes). Expression profile and relationship with GnRHs expression and plasma sex steroid levels in male fish. Journal of Fish Biology. 71: 1-19. Impact Factor: 1,734 SCR indicator: Marine & Freshwater Biology Q1 0,41

11. Kah O., Lethimonier C., Somoza G., Guilgur L.G., Vaillant C., Lareyre J.J. 2007. GnRH
and GnRH receptors in Metazoa: A historical, comparative and evolutive perspective. Ge- neral and Comparative Endocrinology. 153(1-3): 346-64. Impact Factor: 2,674 SCR indi- cator: Endocrinology Q2 1,185

12. Guilgur L.G., Moncaut N.P., Canário A.V.M., Somoza G.M. 2006. Evolution of GnRH ligands and receptors in Gnathostomata. Comparative Biochemistry and Physiology 144: 272-283. Impact Factor: 2,371 SCR indicator: Physiology Q2 0,947

13. Fernandino J.I., Guilgur L.G., Somoza G.M. 2006. Characterization of Dmrt1 cDNA and expression during spermatogenesis in the pejerrey, Odontesthes bonariensis. Fish Physio- logy and Biochemistry 32: 231–240. Impact Factor: 1,676 SCR indicator: Physiology Q3 0,692

14. Somoza G.M., Miranda L.A., Guilgur L.G., Strobl-Mazzulla P.H. 2006. Characterization of the brain-pituitary axis in pejerrey Odontesthes bonariensis. Biocell 30: 89-95. Impact Factor: 0,73 SCR indicator: Cell biology Q4 0,228

15. Mongiat L., Fernández M.O., Lux-Lantos V.A.R., Guilgur L.G., Somoza G.M., Libertun C. 2006. Experimental data supporting the expression of the highly conserved GnRH-II in the brain and pituitary gland of rats. Regulatory Peptides 136: 50-57. Impact Factor: 2,014 SCR indicator: Endocrinology Q2 0,848

16. Fernandino J.L., Guilgur L.G., Strobl-Mazzulla P.H., Somoza G.M. 2003. Molecular clo- ning of SOX9, DMRT1 and SF1 cDNA partial sequences in the pejerrey fish Odontesthes bonariensis (Atheriniformes) Fish Physiol. Biochem. 28: 145-146. Impact Factor: 1,676 SCR indicator: Physiology Q3 0,692

17. Guilgur L.G., Miranda L.A., Somoza G.M. 2003. Characterization of three GnRH cDNA sequences in the pejerrey fish Odontesthes bonariensis. Fish Physiol. Biochem. 28: 39-40. Impact Factor: 1,676 SCR indicator: Physiology Q3 0,692

18. Somoza G.M., Miranda L.A., Strobl-Mazzulla P.H., Guilgur L.G. 2002. Gonadotropin-re- leasing hormone (GnRH): from fish to mammalian brains. Cellular and Molecular Neuro- biology 22:589-609. Impact Factor: 2,201 SCR indicator: Cellular and Molecular Neu- roscience. Q4 0,968
2 Protocols published
Protein Extraction from Drosophila Embryos and Ovaries
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 18248, Q&A: 0
Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos ...
FLP/FRT Induction of Mitotic Recombination in Drosophila Germline
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 28011, Q&A: 0
The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces ...
8 Protocols reviewed
RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus
Authors:  Kazuki Sakura, Shinichi Morita and Teruyuki Niimi, date: 04/20/2022, view: 1231, Q&A: 0

In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis. Their functions were investigated using RNA interference (RNAi) method,

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Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
Authors:  Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross, date: 06/05/2021, view: 2756, Q&A: 0

Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the

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1 Protocol edited
A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
Authors:  Kunal Chakraborty, K. VijayRaghavan and Rajesh Gunage, date: 05/20/2018, view: 7984, Q&A: 0
Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury ...
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