Education

Ph.D. in Pharmacy, Department of Botany, Complutense University of Madrid, Spain, 1972

Current position

Professor Emeritus at the CSIC, Department of Plant Biochemistry, Cell and Molecular Biology, Estación Experimental del Zaidín, Granada, Spain

Publications (since 2005)

1. Zienkiewicz, A., Zienkiewicz, K. and Rodríguez-García, M. I. (2014). Storage lipids in developing and germinating pollen grain of flowering plants. Reproductive Biology of Plants: 133.
   
2. Zienkiewicz, A., Rejon, J. D., de Dios Alche, J., Rodríguez-García, M. I. and Castro, A. J. (2014). A protocol for protein extraction from lipid-rich plant tissues suitable for electrophoresis. Methods Mol Biol 1072: 85-91.
   
3. Zienkiewicz, A., Zienkiewicz, K., Rejon, J. D., Alche Jde, D., Castro, A. J. and Rodríguez-García, M. I. (2014). Olive seed protein bodies store degrading enzymes involved in mobilization of oil bodies. J Exp Bot 65(1): 103-115.
   
4. Soleimani, A., Morales, S., Jimenez-Lopez, J. C., Castro, A. J., Rodríguez-García, M. I. and de Dios Alche, J. (2013). Differential expression and sequence polymorphism of the olive pollen allergen Ole e 1 in two Iranian cultivars. Iran J Allergy Asthma Immunol 12(1): 18-28.
   
5. Ribeiro, H., Morales, S., Salmerón, C., Cruz, A., Calado, L., Rodríguez-García, M., Alché, J. and Abreu, I. (2013). Analysis of the pollen allergen content of twelve olive cultivars grown in Portugal. Aerobiologia 29(4): 513-521.
   
6. Marco, F. M., Morales, S., Castro, A. J., Salmeron, C., Rodríguez-García, M. I. and Alche, J. D. D. (2013). A preliminary proposal for the clustering of olive pollens into model cultivars on the basis of their allergenic content, pag 315. ALLERGY^© Published by John Wiley and Sons Ltd, 68 (Suppl. 97), 189-336.
   
7. Suarez, C., Zienkiewicz, A., Castro, A. J., Zienkiewicz, K., Majewska-Sawka, A. and Rodríguez-García, M. I. (2013). Cellular localization and levels of pectins and arabinogalactan proteins in olive (Olea europaea L.) pistil tissues during development: implications for pollen-pistil interaction. Planta 237(1): 305-319.
   
8. Castro, A. J., Suarez, C., Zienkiewicz, K., Alche Jde, D., Zienkiewicz, A. and Rodríguez-García, M. I. (2013). Electrophoretic profiling and immunocytochemical detection of pectins and arabinogalactan proteins in olive pollen during germination and pollen tube growth. Ann Bot 112(3): 503-513.
   
9. Jimenez-Lopez, J. C., Rodríguez-García, M. I. and Alche, J. D. (2013). Analysis of the effects of polymorphism on pollen profilin structural functionality and the generation of conformational, T- and B-cell epitopes. PLoS One 8(10): e76066.
   
10. Rejon, J. D., Delalande, F., Schaeffer-Reiss, C., Carapito, C., Zienkiewicz, K., de Dios Alche, J., Rodríguez-García, M. I., Van Dorsselaer, A. and Castro, A. J. (2013). Proteomics profiling reveals novel proteins and functions of the plant stigma exudate. J Exp Bot 64(18): 5695-5705.
    
11. Mandi, F., Rodríguez-García, M. I. and Alche, J. D. (2013). Desarrollo y aplicaciones de una técnica genética para detectar fraudes en el aceite de oliva y garantizar su origen varietal. Olimerca 5:46-49
    
12. Zafra, A., Carmona, R., Jimenez-Quesada, M., Traverso, J., Castro, A., Rodríguez-García, M., Bautista, R., Claros, M. and Alche, J. (2013). Transcriptome analysis of enzyme activities regulating ROS metabolism in olive (Olea europaea L.) reproductive tissues. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology 94(2).
    
13. Zienkiewicz, A., Jimenez-quesada, M. J., Traverso, J. A., Zafra, A., Lopez-huertas, E., Zienkiewicz, K., Castro, A. J., Rodríguez-García, M. I. and Alche, J. D. (2013). Enzyme activities regulating ROS metabolism in olive (Olea europaea L.) seeds. BioTechnologia 94(2): 229.
    
14. Traverso, J. A., Pulido, A., Rodríguez-García, M. I. and Alché, J. D. (2013). Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives. Front Plant Sci 4.
    
15. Jimenez-Lopez, J. C., Morales, S., Castro, A. J., Volkmann, D., Rodríguez-García, M. I. and Alche, J. D. (2012). Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS One 7(2): e30878.
    
16. Morales, S., Castro, A. J., Jimenez-Lopez, J. C., Florido, F., Rodríguez-García, M. I. and de Dios Alche, J. (2012). A novel multiplex method for the simultaneous detection and relative quantitation of pollen allergens. Electrophoresis 33(9-10): 1367-1374.
    
17. Jimenez-Lopez, J. C., Kotchoni, S. O., Rodríguez-García, M. I. and Alche, J. D. (2012). Structure and functional features of olive pollen pectin methylesterase using homology modeling and molecular docking methods. J Mol Model 18(12): 4965-4984.
    
18. Suarez, C., Castro, A. J., Rapoport, H. F. and Rodríguez-García, M. I. (2012). Morphological, histological and ultrastructural changes in the olive pistil during flowering. Sex Plant Reprod 25(2): 133-146.
    
19. Rodríguez-García, M. I., Alche, J. D., Castro, A. J., Suarez, C. and Rejon, J. D. (2012). Pollen and Pistil Features Involved in the Reproductive Biology of the Olive (Olea Europaea L.) In: Palmer, J., Herrero, M., Hormaza, I., Tustin, S., Talón, M., Tadeo, F. R., Chaves, M. and Webster, A. D. (eds). Plant physiology from cell to fruit production system (Provisional Title). Acta Horticulturae, ACTA HORTICULTURAE (ISHS), Vol I-H09, 932:83-89.
    
20. Zafra, A., Jiménez-Quesada, M., Traverso, J., Corpas, F., Rodríguez-García, M. and Alché, J. (2012). Peroxisomal localization of CuZn superoxide dismutase in the male reproductive tissues of the olive tree. Microscopy and Microanalysis 18(S5): 33-34.
    
21. Rejon, J. D., Zienkiewicz, A., Rodríguez-García, M. I. and Castro, A. J. (2012). Profiling and functional classification of esterases in olive (Olea europaea) pollen during germination. Ann Bot 110(5): 1035-1045.
    
22. Jimenez-lopez, J. C., Kotchoni, S. O., Gachomo, E. W., Castro, A. J., Rodríguez-García, M. I. and Alche, J. D. (2012). Molecular features of maize allergens and their implications in human health. In: Jimenez-Lopez, J. C. (ed). Maize: Cultivation, Uses And Health Benefits. Nova Science Publishers, 137-178.
    
23. Alche, J. D., Castro, A. J., Jimenez-lopez, J. C., Morales, S., Zafra, A., Florido, F., Maria, I. and Rodríguez-García, M. I. (2012). Pollen allergenicity is highly dependent on the plant genetic background: the “cultivar” issue”. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. Intech Open Science, 1-26.
    
24. Morales, S., Castro, A. J., Salmerón, C., Marco de la Calle, F. M., Rodríguez-García, M. I. and Alché, J. d. D. (2012). Clustering of olive pollens into model cultivars on the basis of their allergenic content, Intech.
    
25. Zienkiewicz, K., Garcia-quiros, E., Alche, J. D., Rodríguez-García, J. D. and Castro, A. J. (2012). Simultaneous detection and quantitation of pollen allergens using a fluorescence-based 2-D multiplex approach. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens.  Intech Open Science, 41-56.
    
26. Morales, S., Castro, A. J., Rodríguez-García, M. I. and Alche, J. D. (2012). Involvement of climatic factors over the allergen expression in olive pollen. In: Jiménez-López, J. C. (ed). Current Insights in Pollen Allergens. Intech Open Science, 57-70.
    
27. Jimenez-Lopez, J. C., Morales, S., Volkmann, D., Alche, J. D. and Rodríguez-García, M. I. (2012). Differential immune-reactivity and subcellular distribution reveal the multifunctional character of pollen profilins as major effect of sequences polymorphism. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. Intech Open Science, 71-102.
    
28. Zienkiewicz, A., Jiménez-López, J. C., Zienkiewicz, K., de Dios Alché, J. and Rodríguez-García, M. I. (2011). Development of the cotyledon cells during olive (Olea europaea L.) in vitro seed germination and seedling growth. Protoplasma 248(4): 751-765.
    
29. Jiménez-López, J. C., Rodríguez-García, M. I. and de Dios Alche, J. (2011). Systematic and phylogenetic analysis of the Ole e 1 pollen protein family members in plants. In: Yang, N. S. (ed). Systems and computational biology: Bioinformatics and computational modeling. InTech, 12:245-260.
30. Zienkiewicz, K., Zienkiewicz, A., Rodríguez-García, M. I. and Castro, A. J. (2011). Characterization of a caleosin expressed during olive (Olea europaea L.) pollen ontogeny. BMC Plant Biol 11: 122.
    
31. Zienkiewicz, K., Rejon, J. D., Suarez, C., Castro, A. J., de Dios Alche, J. and Rodríguez-García, M. I. (2011). Whole-organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant reproduction. BMC Plant Biol 11: 150.
    
32. Iglesias, V. A.-V., Alonso, S. B., Blanco, J. L. S., Montaña, P. G., García, M. I. R. and Rodríguez, M. C. M. (2011). Leaf thickness and structure of" Vitis vinifera" L. cv. Albariño clones and its possible relation with susceptibility to downy mildew (" Plasmopara viticola") infection. Inter J of Vine Wine Sci 45(3): 161-169.
    
33. Castro, A. J., Bednarczyk, A., Schaeffer‐Reiss, C., Rodríguez‐García, M. I., Van Dorsselaer, A. and de Dios Alché, J. (2010). Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N‐glycopeptide analysis. Proteomics 10(5): 953-962.
    
34. Alché Ramírez, J. d. D., Olmedilla, A. and Rodríguez García, M. I. (2010). Análisis estructural del aparato fotosintético en plantas C4 y CAM mediante microscopía de fluorescencia/confocal.
    
35. Zafra, A., Rodriguez-Garcia, M. I. and Alche Jde, D. (2010). Cellular localization of ROS and NO in olive reproductive tissues during flower development. BMC Plant Biol 10: 36.
    
36. Zienkiewicz, K., Castro, A. J., Alche Jde, D., Zienkiewicz, A., Suarez, C. and Rodríguez-García, M. I. (2010). Identification and localization of a caleosin in olive (Olea europaea L.) pollen during in vitro germination. J Exp Bot 61(5): 1537-1546.
    
37. Morales, S., Rodríguez-García, M. I. and Alché Ramírez, J. d. D. (2010). Advances in multilabeling for TEM and CLSM using primary antibodies derived from the same species. Application of Fab fragments to co-localize olive pollen allergens.
    
38. Castro López, A. J., Rejón, J. D., Fendri, M., Jiménez-Quesada, M. J., Zafra, A., Jiménez-López, J. C., Rodríguez García, M. I. and Alché Ramírez, J. d. D. (2010). Taxonomical discrimination of pollen grains by using confocal laser scanning microscopy (CLSM) imaging of autofluorescence.
    
39. Fendri, M., Trujillo, I., Trigui, A., Rodríguez-García, M. I. and Ramírez, J. d. D. A. (2010). Simple sequence repeat identification and endocarp characterization of olive tree accessions in a Tunisian germplasm collection. Hortscience 45(10): 1429-1436.
    
40. Rejon, J. D., Suarez,C. G., Alche, J. D., Castro, A. J. and Rodríguez-García, M. I. (2010). Evaluation of different methods to determine pollen quality in several olive (Olea europaea L.) cultivars. Polen 20: 61-72.
    
41. Alché Ramírez, J. d. D., Rejón, J. D., Suárez, C. and Rodríguez García, M. I. (2009). Reproducción sexual en el olivo. Investigacion y Ciencia 391: 40-41.
42. Rodríguez García, M. I., Rejón, J. D., Alché Ramírez, J. d. D. and Castro López, A. J. (2009). Mesure de la germination in vitro du pollen d´ olivier en utilisant la cytométrie en flux. In: Karray, B., Khecharem, J. and Roussos, S. (eds). Institut de l’olivier.
43. Fendri, M., Trujillo, I., Rodríguez García, M. I. and Alché Ramírez, J. d. D. (2009). Utilisation de marqueurs microsatellites pour la caractérisation de variétés d´ olives tunisiennes. In: Karray, B., Khecharem, J. and Roussos, S. (eds). Institut de l’olivier, 383-385.
    
44. Santos, M., Alché Ramírez, J. d. D., Rodríguez García, M. I. and Torné, J. (2007). Transglutaminase activity and localization during microspore induction in maize.
    
45. Morales, S., Jimenez-Lopez, J. C., Castro, A. J., Rodríguez-García, M. I. and Alche, J. D. (2008). Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination. J Microsc 231(2): 332-341.
    
46. Hamman-Khalifa, A., Castro, A. J., Jimenez-Lopez, J. C., Rodríguez-García, M. I. and Alche Jde, D. (2008). Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen. BMC Plant Biol 8: 10.
    
47. Serrano, I., Suarez, C., Olmedilla, A., Rapoport, H. and Rodríguez-García, M. I. (2008). Structural organization and cytochemical features of the pistil in olive (Olea europaea L. var. Picual) at anthesis. Sexual Plant Reprod 21:99–111
    
48. Zienkiewicz, K., Zienkiewicz, A., Rodríguez-García, M. I., Smolinski, D. J., Swidzinski, M. and Bednarska, E. (2008). Transcriptional activity and distribution of splicing machinery elements during Hyacinthus orientalis pollen tube growth. Protoplasma 233(1-2): 129-139.
    
49. Wang, W., de Dios Alché, J. and Rodríguez-García, M. I. (2007). Characterization of olive seed storage proteins. Acta Physiologiae Plantarum 29(5): 439-444.
    
50. Alché Ramírez, J. d. D., Castro López, A. J., Jiménez-López, J. C., Morales, S., Zafra, A., Hamman-Khalifa, A. M. and Rodríguez García, M. I. (2007). Differential characteristics of olive pollen from different cultivars: biological and clinical implications.
    
51. Castro López, A. J., Alché Ramírez, J. d. D., Calabozo, B., Rodríguez García, M. I. and Polo, F. (2007). Pla l 1 and Ole e 1 pollen allergens share common epitopes and similar ultrastructural localization. J Invest Allergology Clin 17(S1): 93-99.
    
52. Jimenez-Lopez, J. C., Alche, J. D., Wang, W. and Rodríguez-García, M. I. (2007). El contenido proteico mayoritario del alpeorujo corresponde a proteínas de almacenamiento de tipo 11S. In: EL ACEITE DE OLIVA. Tomo 1, 365-376.
    
53. Majewska-Sawka, A. and Rodríguez-García, M. I. (2006). Immunodetection of pectin and arabinogalactan protein epitopes during pollen exine formation of Beta vulgaris L. Protoplasma 228(1-3): 41-47.
    
54. de Dios Alche, J., Jimenez-Lopez, J. C., Wang, W., Castro-Lopez, A. J. and Rodríguez-García, M. I. (2006). Biochemical characterization and cellular localization of 11S type storage proteins in olive (Olea europaea L.) seeds. J Agric Food Chem 54(15): 5562-5570.
    
55. Barral, P., Suarez, C., Batanero, E., Alfonso, C., Alche Jde, D., Rodríguez-García, M. I., Villalba, M., Rivas, G. and Rodriguez, R. (2005). An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination. Biochem J 390(Pt 1): 77-84.