Dear Bahtiyar,
Unfortunately, we have not tried purifying AsCas12a without MBP tag.
We have purified FnCas12a and other Cas12a variants with and without MBP tag, and did not see any significant changes in yield. Although this does not give any guarantees for AsCas12a, I think it is worth trying.
With kind regards,
Daan
2018-10-26 02:59:25