Toshiyuki Habu Radiation Biology Center, Kyoto University, Japan
1 protocol
Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
57 protocols

Anna Zorina Russian Academy of Sciences
5 protocols

Antoine de Morree Stanford university
37 protocols

Arsalan Daudi University of California
157 protocols

Tatsuki Kunoh
  • Okayama University
Research focus
  • Environmental science
  • 1 Author merit


Ph.D. in Department of Biotechnology, Graduate School of Engineering, Osaka University, 2000

Current position

Lecturer in Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan


  1. Kunoh, T. and Habu, T. (2014). Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity. Springerplus 3: 30.
  2. Hagihara, K., Mizukura, A., Kitai, Y., Yao, M., Ishida, K., Kita, A., Kunoh, T., Masuko, T., Matzno, S., Chiba, K. and Sugiura, R. (2014). FTY720 stimulated ROS generation and the Sty1/Atf1 signaling pathway in the fission yeast Schizosaccharomyces pombe. Genes Cells 19(4): 325-337.
  3. Hasegawa, M., Yasuda, Y., Tanaka, M., Nakata, K., Umeda, E., Wang, Y., Watanabe, C., Uetake, S., Kunoh, T., Shionyu, M., Sasaki, R., Shiina, I. and Mizukami, T. (2014). A novel tamoxifen derivative, ridaifen-F, is a nonpeptidic small-molecule proteasome inhibitor. Eur J Med Chem 71: 290-305.
  4. Hagihara, K., Kita, A., Mizukura, A., Yao, M., Kitai, Y., Kunoh, T., Masuko, T., Matzno, S., Chiba, K. and Sugiura, R. (2013). Fingolimod (FTY720) stimulates ca(2+)/calcineurin signaling in fission yeast. PLoS One 8(12): e81907.
  5. Sugiura, R., Kita, A., Tsutsui, N., Muraoka, O., Hagihara, K., Umeda, N., Kunoh, T., Takada, H. and Hirose, D. (2012). Acremomannolipin A, the potential calcium signal modulator with a characteristic glycolipid structure from the filamentous fungus Acremonium strictum. Bioorg Med Chem Lett 22(21): 6735-6739.
  6. Mizukami, T., Kunoh, T. and Sasaki, R. (2012). Discovery of drug targets and seeds by 'humanized yeast' technology. Nihon Rinsho 70 Suppl 8: 308-315.
  7. Hasegawa, M., Miura, T., Kuzuya, K., Inoue, A., Won Ki, S., Horinouchi, S., Yoshida, T., Kunoh, T., Koseki, K., Mino, K., Sasaki, R., Yoshida, M. and Mizukami, T. (2011). Identification of SAP155 as the target of GEX1A (Herboxidiene), an antitumor natural product. ACS Chem Biol 6(3): 229-233.
  8. Kunoh, T., Noda, T., Koseki, K., Sekigawa, M., Takagi, M., Shin-ya, K., Goshima, N., Iemura, S., Natsume, T., Wada, S., Mukai, Y., Ohta, S., Sasaki, R. and Mizukami, T. (2010). A novel human dynactin-associated protein, dynAP, promotes activation of Akt, and ergosterol-related compounds induce dynAP-dependent apoptosis of human cancer cells. Mol Cancer Ther 9(11): 2934-2942.
  9. Sekigawa, M., Kunoh, T., Wada, S., Mukai, Y., Ohshima, K., Ohta, S., Goshima, N., Sasaki, R. and Mizukami, T. (2010). Comprehensive screening of human genes with inhibitory effects on yeast growth and validation of a yeast cell-based system for screening chemicals. J Biomol Screen 15(4): 368-378.
  10. Ueda, J. Y., Kunoh, T., Sekigawa, M., Wada, S., Mukai, Y., Ohta, S., Sasaki, R., Mizukami, T., Takagi, M. and Shin-ya, K. (2010). JBIR-14, a highly oxygenated ergostane, from Isaria sp. NBRC 104353. J Antibiot (Tokyo) 63(3): 139-141.
  11. Kunoh, T., Habu, T. and Matsumoto, T. (2008). Involvement of fission yeast Clr6-HDAC in regulation of the checkpoint kinase Cds1. Nucleic Acids Res 36(10): 3311-3319.
  12. Shimura, T., Toyoshima, M., Adiga, S. K., Kunoh, T., Nagai, H., Shimizu, N., Inoue, M. and Niwa, O. (2006). Suppression of replication fork progression in low-dose-specific p53-dependent S-phase DNA damage checkpoint. Oncogene 25(44): 5921-5932.
  13. Kunoh, T., Kaneko, Y. and Harashima, S. (2000). Positive regulation of transcription of homeoprotein-encoding YHP1 by the two-component regulator Sln1 in Saccharomyces cerevisiae. Biochem Biophys Res Commun 278(2): 344-348.
  14. Kunoh, T., Sakuno, T., Furukawa, T., Kaneko, Y. and Harashima, S. (2000). Genetic characterization of rbt mutants that enhance basal transcription from core promoters in Saccharomyces cerevisiae. J Biochem 128(4): 575-584.
  15. Kunoh, T., Kaneko, Y. and Harashima, S. (2000). YHP1 encodes a new homeoprotein that binds to the IME1 promoter in Saccharomyces cerevisiae. Yeast 16(5): 439-449.
  16. Mizuno, T., Nakazawa, N., Remgsamrarn, P., Kunoh, T., Oshima, Y. and Harashima, S. (1998). The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae. Curr Genet 33(4): 239-247.
1 Protocol published
Chromatin Fractionation Assay in Fission Yeast
Authors:  Tatsuki Kunoh and Toshiyuki Habu, date: 07/20/2014, view: 10970, Q&A: 0
The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a ...
6 Protocols reviewed
Immunostaining of Formaldehyde-fixed Metaphase Chromosome from Untreated and Aphidicolin-treated DT40 Cells
Author:  Vibe H. Oestergaard, date: 05/05/2017, view: 9131, Q&A: 0
During mitosis chromosomes are condensed into dense X-shaped structures that allow for microscopic determination of karyotype as well as inspection of chromosome morphology.

This protocol describes a method to perform immunostaining of ...
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ARP2/3 Phosphorylation Assay in the Presence of Recombinant Bacterial Effectors
Authors:  Céline Michard, Lawrence L. LeClaire and Patricia Doublet, date: 04/05/2017, view: 7403, Q&A: 0
The Actin-Related Protein 2/3 (ARP2/3) complex is an actin nucleator that generates a branched actin network in mammalian cells. In addition to binding nucleation promoting factors, LeClaire et al. demonstrated that its phosphorylation ...
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