Jean Grivel Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH, USA
1 protocol

Christophe Vanpouille Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH, USA
1 protocol

Andrea Introini Karolinska Institutet
1 protocol

Leonid Margolis
  • Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH, USA
Research focus
  • Microbiology
  • 1 Author merit


Ph.D. in Oncology, Moscow State University, Russia, 1974
Dr. Sci (advanced degree) in Biophysics, Cell Biology, Moscow State University, Russia, 1985

Current position

Active Deputy Director, NASA/NIH Center for Three-Dimensional Tissue Culture, 1995
Active Senior Investigator, NICHD, NIH, 2000
Active Chief, Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH, 2001


Selected peer-reviewed publications (out of 213 total)

  1. Introini, A., Vanpouille, C., Lisco, A., Grivel, J. C. and Margolis, L. (2013). Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo. PLoS Pathog 9(2): e1003148.
  2. Andrei, G., Lisco, A., Vanpouille, C., Introini, A., Balestra, E., van den Oord, J., Cihlar, T., Perno, C. F., Snoeck, R., Margolis, L. and Balzarini, J. (2011). Topical tenofovir, a microbicide effective against HIV, inhibits herpes simplex virus-2 replication. Cell Host Microbe 10(4): 379-389.
  3. Saba, E., Grivel, J. C., Vanpouille, C., Brichacek, B., Fitzgerald, W., Margolis, L. and Lisco, A. (2010). HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model. Mucosal Immunol 3(3): 280-290.
  4. Lisco, A., Vanpouille, C. and Margolis, L. (2009). War and peace between microbes: HIV-1 interactions with coinfecting viruses. Cell Host Microbe 6(5): 403-408.
  5. Grivel, J. C. and Margolis, L. (2009). Use of human tissue explants to study human infectious agents. Nat Protoc 4(2): 256-269.
  6. Lisco, A., Vanpouille, C., Tchesnokov, E. P., Grivel, J. C., Biancotto, A., Brichacek, B., Elliott, J., Fromentin, E., Shattock, R., Anton, P., Gorelick, R., Balzarini, J., McGuigan, C., Derudas, M., Gotte, M., Schinazi, R. F. and Margolis, L. (2008). Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues. Cell Host Microbe 4(3): 260-270.
  7. Munch, J., Rucker, E., Standker, L., Adermann, K., Goffinet, C., Schindler, M., Wildum, S., Chinnadurai, R., Rajan, D., Specht, A., Gimenez-Gallego, G., Sanchez, P. C., Fowler, D. M., Koulov, A., Kelly, J. W., Mothes, W., Grivel, J. C., Margolis, L., Keppler, O. T., Forssmann, W. G. and Kirchhoff, F. (2007). Semen-derived amyloid fibrils drastically enhance HIV infection. Cell 131(6): 1059-1071.
  8. Margolis, L. and Shattock, R. (2006). Selective transmission of CCR5-utilizing HIV-1: the 'gatekeeper' problem resolved? Nat Rev Microbiol 4(4): 312-317.
  9. Margolis, L. (2003). Cytokines--strategic weapons in germ warfare? Nat Biotechnol 21(1): 15-16.
  10. Grivel, J. C., Ito, Y., Faga, G., Santoro, F., Shaheen, F., Malnati, M. S., Fitzgerald, W., Lusso, P. and Margolis, L. (2001). Suppression of CCR5- but not CXCR4-tropic HIV-1 in lymphoid tissue by human herpesvirus 6. Nat Med 7(11): 1232-1235.
  11. Malkevich, N., Womack, C., Pandya, P., Grivel, J. C., Fauci, A. S. and Margolis, L. (2001). Human immunodeficiency virus type 1 (HIV-1) non-B subtypes are similar to HIV-1 subtype B in that coreceptor specificity is a determinant of cytopathicity in human lymphoid tissue infected ex vivo. J Virol 75(21): 10520-10522.
  12. Glushakova, S., Yi, Y., Grivel, J. C., Singh, A., Schols, D., De Clercq, E., Collman, R. G. and Margolis, L. (1999). Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo. J Clin Invest 104(5): R7-R11.
  13. Grivel, J. C. and Margolis, L. B. (1999). CCR5- and CXCR4-tropic HIV-1 are equally cytopathic for their T-cell targets in human lymphoid tissue. Nat Med 5(3): 344-346.
  14. Penn, M. L., Grivel, J. C., Schramm, B., Goldsmith, M. A. and Margolis, L. (1999). CXCR4 utilization is sufficient to trigger CD4+ T cell depletion in HIV-1-infected human lymphoid tissue. Proc Natl Acad Sci U S A 96(2): 663-668.
  15. Glushakova, S., Grivel, J. C., Fitzgerald, W., Sylwester, A., Zimmerberg, J. and Margolis, L. B. (1998). Evidence for the HIV-1 phenotype switch as a causal factor in acquired immunodeficiency. Nat Med 4(3): 346-349.
  16. Margolis, L. B., Glushakova, S., Grivel, J. C. and Murphy, P. M. (1998). Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines. J Clin Invest 101(9): 1876-1880.
  17. Glushakova, S., Baibakov, B., Margolis, L. B. and Zimmerberg, J. (1995). Infection of human tonsil histocultures: a model for HIV pathogenesis. Nat Med 1(12): 1320-1322.
  18. Margolis, L. B., Tikhonov, A. N. and Vasilieva, E. Y. (1980). Platelet adhesion to fluid and solid phospholipid membranes. Cell 19(1): 189-195.
  19. Margolis, L. B., Vasilieva, E. J., Vasiliev, J. M. and Gelfand, I. M. (1979). Upper surfaces of epithelial sheets and of fluid lipid films are nonadhesive for platelets. Proc Natl Acad Sci U S A 76(5): 2303-2305.
  20. Ivanova, O. Y. and Margolis, L. B. (1973). The use of phospholipid film for shaping cell cultures. Nature 242(5394): 200-201.
1 Protocol published
An ex vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue
Authors:  Andrea Introini, Christophe Vanpouille, Jean Charles Grivel and Leonid Margolis, date: 02/20/2014, view: 14349, Q&A: 0
Human tissue explants are a valuable tool to study the interactions between host and infectious agents. They reliably mimic many important aspects of tissue cytoarchitecture and functions and allow us the investigation of the mechanisms of microbial ...
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