Elmon Schmelzer Central Microscopy Unit, Max Planck Institute for Plant Breeding Research, Germany
1 protocol
Ashish Ranjan Ashish Ranjan
3 protocols

Demosthenis Chronis Olea Estates LLC
10 protocols

José O’Brien Pontificia Universidad Católica de Chile Santiago
1 protocol

Qiangjun Zhou Stanford University, Howard Hughes Medical Institute
6 protocols

Reviewer
Malgorzata Lichocka
  • Research Associate, Institute of Biochemistry and Biophysics PAS
Research focus
  • Plant science
  • 2 Author merit

Education

Ph. D. in Agriculture and Biology, Department of Botany, Faculty of Agriculture, Warsaw University of Life Science in Poland, 2006

Current position

Leader of Laboratory of Confocal Microscopy, Institute of Biochemistry and Biophysics, Warsaw, Poland (since 2010)
Scientific collaboration with Laboratory of Plant Pathogenesis, Institute of Biochemistry and Biophysics, Warsaw, Poland (since 2008)

Publications

  1. Hoser, R., Zurczak, M., Lichocka, M., Zuzga, S., Dadlez, M., Samuel, M. A., Ellis, B. E., Stuttmann, J., Parker, J. E., Hennig, J. and Krzymowska, M. (2013). Nucleocytoplasmic partitioning of tobacco N receptor is modulated by SGT1. New Phytol 200(1): 158-171. (IF= 6.736).
  2. Giska, F., Lichocka, M., Piechocki, M., Dadlez, M., Schmelzer, E., Hennig, J. and Krzymowska, M. (2013). Phosphorylation of HopQ1, a type III effector from Pseudomonas syringae, creates a binding site for host 14-3-3 proteins. Plant Physiol 161(4): 2049-2061. (IF=7.016).
  3. Nowicki, M., Lichocka, M., Nowakowska, M., Klosińska, U. and Kozik, E. U. (2012). A Simple Dual Stain for Detailed Investigations of Plant-Fungal Pathogen Interactions. Vegetable Crops Research Bulletin 77(1): 61-74. (IF=0.296).
  4. Karczmarek, A., Fudali, S., Lichocka, M., Sobczak, M., Kurek, W., Janakowski, S., Roosien, J., Golinowski, W., Bakker, J., Goverse, A. and Helder, J. (2008). Expression of two functionally distinct plant endo-beta-1,4-glucanases is essential for the compatible interaction between potato cyst nematode and its hosts. Mol Plant Microbe Interact 21(6): 791-798. (IF=4.410).
  5. Lichocka, M. and Golinowski, W. (2008). Two tomato endoglucanases have a function during syncytium development. Acta Societatis Botanicorum Poloniae 77(1): 5-15. (IF=0.476).
2 Protocols published
Biolistic Bombardment for Co-expression of Proteins Fused to YFP and mRFP in Leaf Epidermal Cells of Phaseolus vulgaris ‘Red Mexican’
Author:  Malgorzata Lichocka, date: 01/05/2014, view: 12216, Q&A: 0
Biolistic bombardment is based on coating of tungsten or gold particles with DNA and delivery of these “biobullets” into living plant cells under high pressure (Sudowe and Reske-Kunz, 2013). This method enables transient expression of a DNA ...
Subcellular Localization Experiments and FRET-FLIM Measurements in Plants
Authors:  Malgorzata Lichocka and Elmon Schmelzer, date: 01/05/2014, view: 15598, Q&A: 0
Determining the localization of proteins within living cells may be very essential for understanding their biological function. Usually for analysis of subcellular localization, a construct encoding the translational fusion of a cDNA of interest ...
3 Protocols reviewed
Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
Authors:  Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu, date: 04/20/2022, view: 1148, Q&A: 0

The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed

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Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
Authors:  Liping Wang, Gang Yu, Alberto P. Macho and Rosa Lozano-Durán, date: 12/05/2021, view: 2436, Q&A: 0

The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein

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