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Alizée Malnoë Umeå Plant Science Centre
4 protocols

Ana Espinosa-Ruiz Instituto de Biología Molecular y Celular de Plantas
1 protocol

Annis Richardson University of California, Berkeley
5 protocols

Arsalan Daudi University of California
156 protocols

Editor
Renate Weizbauer
  • Post-Doc, Carnegie Institution for Science
Research focus
  • Plant science
  • 0 Author merit

Education

Ph.D., Purdue University

14 Protocols reviewed
Plant Tissue Trypan Blue Staining During Phytopathogen Infection
In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and ...
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A Ribosome Footprinting Protocol for Plants
Authors:  Catharina Merchante, Qiwen Hu, Steffen Heber, Jose Alonso and Anna N. Stepanova, date: 11/05/2016, view: 4229, Q&A: 0
Ribosome footprinting, or Ribo-seq, has revolutionized the studies of translation. It was originally developed for yeast and mammalian cells in culture (Ingolia et al., 2009). Herein, we describe a plant-optimized hands-on ribosome ...
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23 Protocols edited
Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Authors:  Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 771, Q&A: 0
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...
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In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts
Authors:  Joo Yong Kim and Hak Soo Seo, date: 04/05/2018, view: 640, Q&A: 0
Nitrate reductase (NR) reduces the major plant nitrogen source, NO3-, into NO2-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present ...
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