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Guillermo Pérez
Departement of Medical Sciences, University of Girona, Spain
1 protocol
Fabiana Scornik
University of Girona
2 protocols
Ramon Brugada
Cardiovascular Genetics Center & Medical Sciences Department, Girona Biomedical Research Institute (IDIBGI) & University of Girona School of Medicine, Spain
2 protocols
Anna Tarradas
Cardiovascular Genetics Center, Girona Biomedical Research Institute (IDIBGI) & University of Girona, Spain
2 protocols
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Elisabet Selga
Cardiovascular Genetics Center & Medical Sciences Department, Girona Biomedical Research Institute (IDIBGI) & University of Girona, Spain
Research focus
Cell biology
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2
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2 Protocols published
Sodium Current Measurements in HEK293 Cells
Authors:
Helena Riuró
,
Anna Tarradas
,
Elisabet Selga
,
Ramon Brugada
,
Fabiana Scornik
and
Guillermo Pérez
,
date:
08/20/2013,
view:
12807,
Q&A:
0
This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Na
...
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Cell Surface Protein Biotinylation and Analysis
Authors:
Anna Tarradas
,
Elisabet Selga
,
Helena Riuró
,
Fabiana S. Scornik
,
Ramon Brugada
and
Marcel Vergés
,
date:
08/20/2013,
view:
29481,
Q&A:
0
A great way to specifically isolate and quantify proteins in the cell surface membrane is to take advantage of the biotinylation technique. It consists of labeling cell surface proteins with a biotin reagent before lysing the cells, and isolating ...
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