John Beatty Department of Microbiology and Immunology, University of British Columbia, Vancouver
5 protocols
Indranil Malik University of Michigan
11 protocols

Renate Weizbauer Carnegie Institution for Science
42 protocols

Timo Lehti University of Helsinki
21 protocols

Zhenying Liu University of Pennsylvania
2 protocols

Molly M. Leung
  • University of British Columbia
Research focus
  • Microbiology
  • 3 Author merit


Ph.D. in Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, 2010

Current position

Post-doctorate in Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, California, USA


  1. Leung, M. M., Brimacombe, C. A. and Beatty, J. T. (2013). Transcriptional regulation of the Rhodobacter capsulatus response regulator CtrA. Microbiology 159(Pt 1): 96-106.
  2. Leung, M. M., Brimacombe, C. A., Spiegelman, G. B. and Beatty, J. T. (2012). The GtaR protein negatively regulates transcription of the gtaRI operon and modulates gene transfer agent (RcGTA) expression in Rhodobacter capsulatus. Mol Microbiol 83(4): 759-774.
  3. Leung, M. M., Florizone, S. M., Taylor, T. A., Lang, A. S. and Beatty, J. T. (2010). The gene transfer agent of Rhodobacter capsulatus. Adv Exp Med Biol 675: 253-264.
  4. Tuschak, C., Leung, M. M., Beatty, J. T. and Overmann, J. (2005). The puf operon of the purple sulfur bacterium Amoebobacter purpureus: structure, transcription and phylogenetic analysis. Arch Microbiol 183(6): 431-443.
3 Protocols published
Bacterial Conjugation in Rhodobacter capsulatus
Authors:  Molly M. Leung and John Thomas Beatty, date: 07/05/2013, view: 12258, Q&A: 0
Bacterial conjugation of plasmids is the common method of introducing foreign DNA into Rhodobacter capsulatus because transformational systems have not been shown as efficient methods of introducing DNA to R. capsulatus. For R. ...
Rhodobacter capsulatus Gene Transfer Agent Transduction Assay
Authors:  Molly M. Leung and John Thomas Beatty, date: 02/20/2013, view: 10082, Q&A: 0
The gene transfer agent (GTA) is a bacteriophage-like particle that transfers genomic DNA from a donor to a recipient bacterium. The Rhodobacter capsulatus GTA (RcGTA) was the first to be studied and this protocol has been optimized for ...
2 Protocols reviewed
Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
Authors:  Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 4230, Q&A: 0
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
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Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
Authors:  Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp, date: 10/20/2018, view: 7328, Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...
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