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Salma Merchant
UT Southwestern
2 protocols
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Isabelle Plo-Azevedo
INSERM U1009, Gustave Roussy, France
Research focus
Stem cell
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2
Author merit
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2 Protocols published
Protein Translation Study – Label Protein with S35 Methionine in Cells
Authors:
Salma Hasan
and
Isabelle Plo
,
date:
11/05/2012,
view:
26948,
Q&A:
2
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...
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Jianan Gong
Walter and Eliza Hall institute
Hi, when you maintain your cells in the methionine free media, have you tested whether cells stop making new proteins? Thanks
3/26/2018 10:31:13 PM
Reply
Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead
Authors:
Salma Hasan
and
Isabelle Plo
,
date:
11/05/2012,
view:
15429,
Q&A:
0
The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34
+
cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34
+
...
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