Isabelle Plo-Azevedo INSERM U1009, Gustave Roussy, France
2 protocols
Agnieszka Pastula Uniwerstet Jagiellonski
6 protocols

Doyel Sen E-Scape Bio Inc
4 protocols

Fanglian He Bio-protocol
205 protocols

Gal Haimovich Weizmann Institute of Science Rehovot
132 protocols

Editor
Salma Merchant
  • Post-Doc, UT Southwestern
Research focus
  • Stem cell
  • Hematopoietic Stem cell, Hematopoietic malignancies, Proteostasis, Clonal hematopoiesis, Mitochondria biology
  • 2 Author merit
2 Protocols published
Protein Translation Study – Label Protein with S35 Methionine in Cells
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 26947, Q&A: 2
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...
Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 15429, Q&A: 0
The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34+ cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34+ ...
17 Protocols reviewed
AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells
Authors:  Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff, date: 04/20/2021, view: 2763, Q&A: 0

Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role

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Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
Authors:  Himasha M. Perera and Michael A. Trakselis, date: 06/20/2020, view: 3339, Q&A: 0
The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication ...
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5 Protocols edited
Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
Authors:  Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle, date: 07/20/2022, view: 1165, Q&A: 0

To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the

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Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
Authors:  Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis, date: 06/05/2022, view: 1342, Q&A: 0

Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the

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