中文
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Archive
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
About Us
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Overview
Published
Edited
Reviewed
Following
Feedback
Q&A
Guo N. Huang
Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, USA
Research focus
Immunology
Contributions
3
Author merit
Reviewer merit
Editor merit
3 Protocols published
T Cell Calcium Mobilization Study (Flow Cytometry)
Author:
Guo N. Huang
,
date:
05/05/2012,
view:
14377,
Q&A:
0
Antigen recognition and activation of T cell receptor (TCR) triggers transient calcium release from intracellular compartments and subsequent sustained calcium influx through cell surface
Icrac
channels. Sustained elevation of the ...
More >
Biotinylation of Cell Surface Proteins
Author:
Guo N. Huang
,
date:
05/05/2012,
view:
32770,
Q&A:
1
Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of ...
More >
Gal Haimovich
Weizmann Institute of Science Rehovot
Hi Guo,
What is the importance of the PLO treatment to the plate? Is it just for better adherence of the 293 cells?
thanks
gal
8/30/2018 1:02:46 AM
Reply
Guo Huang
Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, USA
Yes, PLO treatment was used to enhance 293 cell attachment.
2018-08-30 09:37:58
More protocols >
following
More protocols >
0 Protocol feedback
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.