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Caroline Dean
John Innes Centre, Norwich Research Park, United Kingdom
1 protocol
Matthew Hartley
John Innes Centre, Norwich Research Park, United Kingdom
1 protocol
Tjelvar Olsson
John Innes Centre, Norwich Research Park, United Kingdom
1 protocol
Susan Duncan
Earlham Institute, Norwich Research Park, United Kingdom
1 protocol
View all coauthors >
Arsalan Daudi
University of California
157 protocols
Eunsook Park
University of Wyoming
3 protocols
Marisa Rosa
Bio-protocol.org
71 protocols
Pablo Bolanos-Villegas
University of Costa Rica
12 protocols
View all coreviewers >
Stefanie Rosa
SLU
Research focus
Cell biology
Contributions
2
Author merit
Reviewer merit
Editor merit
Publications
Rosa, S., Duncan, S. and Dean, C. (2016).
Mutually exclusive sense-antisense transcription at FLC facilitates environmentally induced gene repression.
Nat Commun
7: 13031.
Duncan, S., Olsson, T. S., Hartley, M., Dean, C. and Rosa, S. (2016).
A method for detecting single mRNA molecules in Arabidopsis thaliana.
Plant Methods
12: 13.
Zhu, D., Rosa, S. and Dean, C. (2015).
Nuclear organization changes and the epigenetic silencing of FLC during vernalization.
J Mol Biol
427(3): 659-669.
Rosa, S., Ntoukakis, V., Ohmido, N., Pendle, A., Abranches, R. and Shaw, P. (2014).
Cell differentiation and development in
Arabidopsis
are associated with changes in histone dynamics at the single-cell level.
Plant Cell
26(12): 4821-4833.
Podufall, J., Tian, R., Knoche, E., Puchkov, D., Walter, A. M., Rosa, S., Quentin, C., Vukoja, A., Jung, N., Lampe, A., Wichmann, C., Böhme, M., Depner, H., Zhang, Y. Q., Schmoranzer, J., Sigrist, S. J. and Haucke, V. (2014).
A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling.
Cell Rep
7(5): 1417-1425.
Rosa, S., De Lucia, F., Mylne, J. S., Zhu, D., Ohmido, N., Pendle, A., Kato, N., Shaw, P. and Dean, C. (2013).
Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization.
Genes Dev
27(17): 1845-1850.
Rosa, S. and Shaw, P. (2013).
Insights into chromatin structure and dynamics in plants.
Biology (Basel)
2(4): 1378-1410.
Pires, A., Rosa, S., Castanheira, S., Fevereiro, P. and Abranches, R. (2012).
Expression of a recombinant human erythropoietin in suspension cell cultures of
Arabidopsis
, tobacco and
Medicago
.
Plant Cell Tiss Org
.
2 Protocols published
Single Molecule RNA FISH in
Arabidopsis
Root Cells
Authors:
Susan Duncan
,
Tjelvar S. G. Olsson
,
Matthew Hartley
,
Caroline Dean
and
Stefanie Rosa
,
date:
04/20/2017,
view:
13078,
Q&A:
0
Methods that allow the study of gene expression regulation are continually advancing. Here, we present an
in situ
hybridization protocol capable of detecting individual mRNA molecules in plant root cells, thus permitting the accurate ...
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Two-photon Photoactivation to Measure Histone Exchange Dynamics in Plant Root Cells
Authors:
Stefanie Rosa
and
Peter Shaw
,
date:
10/20/2015,
view:
7263,
Q&A:
0
Chromatin-binding proteins play a crucial role in chromatin structure and gene expression. Direct binding of chromatin proteins both maintains and regulates transcriptional states. It is therefore important to study the binding properties of these ...
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2 Protocols reviewed
Direct Visualization and Quantification of the Actin Nucleation and Elongation Events
in vitro
by TIRF Microscopy
Authors:
Yuxiang Jiang
and
Shanjin Huang
,
date:
03/05/2017,
view:
8057,
Q&A:
0
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution
in vitro
. Thanks to the development of various fluorescent probes, we can easily ...
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A Live-imaging, Heat Shock-inducible System to Measure Aux/IAA Degradation Rates in Planta
Authors:
Jessica M. Guseman
,
Jennifer L. Nemhauser
and
Britney L. Moss
,
date:
08/05/2016,
view:
9207,
Q&A:
0
An emerging theme in biology is the importance of cellular signaling dynamics. In addition to monitoring changes in absolute abundance of signaling molecules, many signal transduction pathways are sensitive to changes in temporal properties of ...
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