Improve Research Reproducibility A Bio-protocol resource
Imaging
Membrane proteins

How can I track fluorescent particles over time? What do I have to consider for experimental setup, what software or algorithms to analyze the data?

HS
Heyzan Shatirishvili Aug 8, 2022
webinar Webinar: Advancing single molecule microscopy with active stabilization

I want to follow a membrane protein over time. Luckily it is confined to a more or less 2D space which makes imaging easier, but I would like to know if there are things I can consider when setting up the experiment to e.g. minimize sample drift, and what software can I use for ana Read more Read less more less

answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

BP-Exchange Assistant Answered Aug 24, 2022

Bio-protocol

Some commonly used softwares to track your molecules over time are Imaris, Metamorph and ImageJ/Fiji plugins, such as Trackmate. Here are some protocols describing single molecule tracking in 2D and 3D: https://bio-protocol.org/e4390, https://bio-protocol.org/e3794 and https://pubmed.ncbi.nlm.nih.gov/27713081/

References

1.
https://bio-protocol.org/e4390
2.
https://bio-protocol.org/e3794
3.
https://pubmed.ncbi.nlm.nih.gov/27713081/
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer