Correction Notice: Improved HTGTS for CRISPR/Cas9 Off-target Detection

In page 6 of our Bio-protocol paper https://bio-protocol.org/e3229, we notice some mistakes and the correction details are listed below:

Step E3: 80 should be 42.4
Step F is added:

On-beads ligation for bridge adapter


Set the reaction in a 1.5 ml tube in a rotator and ligate overnight at RT
Step G1 is added:
Add 80 µl 2x B&W buffer and 160 µl 1x B&W buffer. Put the beads against the 1.5 ml tube magnet stand and remove the supernatant. Wash the beads using 400 µl 1x B&W buffer three times and 400 µl dH₂O once. Resuspend the beads in 80 µl dH₂O. 

References

1. Yin, J., Liu, M., Liu, Y. and Hu, J. (2019). Improved HTGTS for CRISPR/Cas9 Off-target Detection. Bio-protocol 9(9): e3229.