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Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood   

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Original research article

A brief version of this protocol appeared in:
Stem Cells
Jun 2013

Abstract

CD34 is a glycosylated cell surface protein and represents a well-known marker for primitive progenitor cells in various organs, especially cord blood, bone marrow and fetal liver. CD34+ progenitor cells are suitable for a series of studies, e.g. cell differentiation, transplantation as well as construction of humanized mouse models. Here, we describe a method to isolate CD34+ cells from the human cord blood and fetal liver.

Keywords: Hematopoietic stem cells, Cord Blood, Fetal liver, Magnetic purification, Hepatic progenitor cells

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Chen, Q. and Chen, J. (2013). Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood. Bio-protocol 3(23): e991. DOI: 10.21769/BioProtoc.991.
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Chanchai Songthaveesin
Mahidol University
What are the different cells between bone marrow stem cells and fetal liver stemm cells?
8/25/2014 1:15:49 AM Reply
Qingfeng Chen
Humanized Mouse Unit, Institute of Molecular and Cell Biology, Singapore

We have not looked into fetal bone marrow stem cells. I assume that the major difference could be that fetal liver stem cells contain hepatic progenitors while bone marrow stem cells might be majorly of hematopoietic lineage.

8/27/2014 10:15:00 PM


Jess Novero
Precision for Medicine
Can you please comment on how many CD34 cells can be isolated from Fetal Livers on average?
3/20/2014 2:47:09 PM Reply
Qingfeng Chen
Humanized Mouse Unit, Institute of Molecular and Cell Biology, Singapore

Depending on the gestation age, the number of CD34 cells recovered ranges from 100 millions (18 wk) to 250 millions (23 wk old) from a fetal liver.

3/23/2014 6:10:05 PM