Hi, In Part IA of your protocol you say to make a 1mg/ml final...

Protocol: Isolation of CD34⁺ Cells from Human Fetal Liver and Cord Blood

Hi, In Part IA of your protocol you say to make a 1mg/ml final concentration of collagenase type IV, but then mention a couple of dilution steps after making the collagenase solution. Does this mean that for the final volume you get to before your 37 degree incubation step (i.e. 2x 40 ml) Read more Read less

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