Home About Us For Authors Submit Protocol Archive
This is a correction notice.See the corrected protocol

Correction: Improved HTGTS for CRISPR/Cas9 Off-target Detection    

Jianhang YinJianhang YinMengzhu LiuMengzhu LiuYang LiuYang LiuJiazhi HuJiazhi Hu
DOI:   10.21769/BioProtoc.3462
Published:   Vol 9, Iss 23, December 05, 2019
twitter facebook linkedin
How to cite Favorites Q&A Share your feedback Cited by

Original research article

A brief version of this protocol appeared in:
Nov 2017

 

In page 6 of our Bio-protocol paper https://bio-protocol.org/e3229, we notice some mistakes and the correction details are listed below:

Step E3: 80 should be 42.4
Step F is added:

On-beads ligation for bridge adapter


Set the reaction in a 1.5 ml tube in a rotator and ligate overnight at RT
Step G1 is added:
Add 80 µl 2x B&W buffer and 160 µl 1x B&W buffer. Put the beads against the 1.5 ml tube magnet stand and remove the supernatant. Wash the beads using 400 µl 1x B&W buffer three times and 400 µl dH2O once. Resuspend the beads in 80 µl dH2O. 

References

  1. Yin, J., Liu, M., Liu, Y. and Hu, J. (2019). Improved HTGTS for CRISPR/Cas9 Off-target Detection. Bio-protocol 9(9): e3229.
Please login/register for free to view full text
Free to view full text
Free to download PDF
Free to question to author
Copyright: © 2019 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Yin, J., Liu, M., Liu, Y. and Hu, J. (2019). Correction: Improved HTGTS for CRISPR/Cas9 Off-target Detection. Bio-protocol 9(23): e3462. DOI: 10.21769/BioProtoc.3462.
Q&A

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data including images for the troubleshooting.

News
Become a Reviewer
Meet Our Ambassadors
twitter facebook linkedin
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Contact Us
For Authors
Submission Procedure
Preparation Guideline
Submit Manuscript
Editorial Process
Editorial Criteria
Competing Interests
Copyright & Permissions
Other Resources
www.bio-101.org for basic protocols

www.bio-thing.com for reagents & equipment

©  Bio-protocol LLC. ISSN: 2331-8325 Advertising Terms and Conditions | Terms of Service | Copyright IP Policy
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.