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The authors used this protocol in:
Nov 2017
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This is a correction notice.See the corrected protocol

Correction: Improved HTGTS for CRISPR/Cas9 Off-target Detection    

Jianhang YinJianhang YinMengzhu LiuMengzhu LiuYang LiuYang LiuJiazhi HuJiazhi Hu
DOI:   10.21769/BioProtoc.3462
Published:   Vol 9, Iss 23, December 05, 2019
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In page 6 of our Bio-protocol paper https://bio-protocol.org/e3229, we notice some mistakes and the correction details are listed below:

Step E3: 80 should be 42.4
Step F is added:

On-beads ligation for bridge adapter


Set the reaction in a 1.5 ml tube in a rotator and ligate overnight at RT
Step G1 is added:
Add 80 µl 2x B&W buffer and 160 µl 1x B&W buffer. Put the beads against the 1.5 ml tube magnet stand and remove the supernatant. Wash the beads using 400 µl 1x B&W buffer three times and 400 µl dH2O once. Resuspend the beads in 80 µl dH2O. 

References

  1. Yin, J., Liu, M., Liu, Y. and Hu, J. (2019). Improved HTGTS for CRISPR/Cas9 Off-target Detection. Bio-protocol 9(9): e3229.
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Copyright: © 2019 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Yin, J., Liu, M., Liu, Y. and Hu, J. (2019). Correction: Improved HTGTS for CRISPR/Cas9 Off-target Detection. Bio-protocol 9(23): e3462. DOI: 10.21769/BioProtoc.3462.
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