Hi,
Thanks for posting this protocol. I find this very helpful for my studies. However, I have following few questions:
1) I will be performing this assay with leaves of Arabidopsis seedlings (12-day old). In that case, which multiwell plate do you think would be best considering the relatively smaller size of seedlings than a fully formed rosette. Can I use a multiwell plate with more number of wells? Can you please tell me which company's multi well plate have you used and the product code as I am calculating the costs for my experiments so it would be very helpful.
2) Steps 20 and 22 require replacing solutions. Do we pour off solutions or pipette them out?
3) After step 22, there is an optional step where we could place our samples at 4degrees in for about 4 days. Do we place the samples in the bleaching solutions for these many days or remove the solution and just place leave samples in plates at 4 degree?
4) Since, I will be cutting the rosette of my seedlings for this assay. Do I need an extra step of placing the samples in water to stabilize the wounding effect that might have arised due to cutting?
Your guidance on this would be very helpful.
Thanks
3/3/2017 11:59:04 PM Reply