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I have followed the Thordal-Christensen's method without using...

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Ren Bing Apr 15, 2013
protocol Protocol: Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
I have followed the Thordal-Christensen's method without using Tween 20 and Na2HPO4 to detect the H2O2 in apple leaves infected by pathogen,but it doesnot work.I want to know the reason that you add Tween 20 and Na2HPO4 to DAB solution. Thank you!
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Arsalan Daudi Author Answered Apr 16, 2013

University of California

Tween 20 aids in the even dispersal and uniform infiltration of DAB. A precisely buffered and pH maintained DAB solution maintains its stability for longer during the assay. Both these features contribute to the robustness and reproducibility of this protocol.
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