Abstract
Generating mouse macrophages from bone-marrow progenitor cells is a useful tool to study biological functions of mouse macrophages. Macrophages are one of the major populations of phagocytes and play many different roles during inflammatory process initiation and termination.
Keywords: Phagocytes, Macrophages, In vitro, M-CSF
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is described in our Nature paper (Zanoni et al., 2009). This work was supported by grants from the CARIPLO Foundation, the European Commission 6th Framework Program (MUGEN and DC-THERA contracts), the European Commission 7th Framework Program (TOLERAGE and ENCITE contracts), the Associazione Italiana per la Ricerca sul Cancro (AIRC) and the and the Italian Ministry of Education and Research (COFIN).
References
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Our lab seed cells in 12-well plates at 1 *10^6 cells per wall concentration. Usually, we change culture medium every 2 days and differentiation may last 7 days.