Improve Research Reproducibility A Bio-protocol resource

I am currently culturing BMDM and following this protocol. I...

 Feb 28, 2012
protocol Protocol: Generation of Mouse Bone Marrow-Derived Macrophages (BM-MFs)
I am currently culturing BMDM and following this protocol. I did the bone marrow isolation yesterday and seeded the cells on 100 mm non-treated culture dishes. After looking at the cells today, there just seems to be a many undifferentiated cells on the plate (they do not have any processe Read more Read less more less
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songzi liu Answered Nov 19, 2018

WuHan University

Our lab seed cells in 12-well plates at 1 *10^6 cells per wall concentration. Usually, we change culture medium every 2 days and differentiation may last 7 days.
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