Abstract
Somatic chromosomes are usually studied from the root tip cells of the plants for cytological investigations. In dioecious plant species like Coccinia grandis, it is very difficult to get meristematic root tip cells from the mature plants of the respective sex forms. In this report, young leaves of the respective sexual phenotypes were used as tissue samples for mitotic chromosome analysis. For meiotic preparation, flower buds of appropriate size were selected for chromosomal studies. Following protocols could be effectively used for routine chromosome preparations in other plant species as well.
Keywords: Sex chromosomes, Mitosis, Meiosis, Dioecious, Coccinia grandis
Materials and Reagents
Equipment
Procedure
Notes
Material should be collected on a bright sunny day to ensure proper mitotic stage development. Cloudy or rainy day should be avoided for material collection.
Recipes
Acknowledgments
Financial support from Department of Biotechnology (DBT), Govt. of India is thankfully acknowledged. Core support from IISER Pune is also acknowledged.
References
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