I am currently culturing BMDM and following this protocol. I did the bone marrow isolation yesterday and seeded the cells on 100 mm non-treated culture dishes. After looking at the cells today, there just seems to be a many undifferentiated cells on the plate (they do not have any processes, just bright, round cells). Is 1 day too early to expect to see any differentiation in the cells? Or might there by something wrong with my media? Any help is appreciated on what the cells are expected to look like 1 day after extraction.
All the best,
Muktha
2/28/2012 8:05:12 PM Reply