微生物学

分类

    现刊
    Purification of Rice Stripe Virus
    水稻条纹病毒纯化
    作者:Gang Lu, Min Yao, Yijun Zhou and Xiaorong Tao日期:03/20/2020,浏览量:152,Q&A: 0
    Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe ...
    Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
    1型单纯疱疹病毒的繁殖,滴定和一步生长曲线
    Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic ...
    Purification of Rice Stripe Virus
    水稻条纹病毒纯化
    作者:Gang Lu, Min Yao, Yijun Zhou and Xiaorong Tao日期:03/20/2020,浏览量:152,Q&A: 0
    [Abstract] Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated ...
    Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria
    革兰阴性菌中内外膜通透性的同期测定
    作者:Bo Ma, Chao Fang, Jing Zhang, Mingzhi Wang, Xiaoxing Luo and Zheng Hou日期:03/05/2020,浏览量:266,Q&A: 0
    [Abstract] The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to ...
    Growth Recovery Assay and FACS-based Population Sorting Following Territorial Exclusion in Proteus mirabilis
    奇异变形杆菌被领土排斥后生长恢复测定和基于FACS的群体分类
    作者:Murray J. Tipping and Karine A. Gibbs日期:03/05/2020,浏览量:253,Q&A: 0
    [Abstract] Many bacteria take part in self recognition and kin discrimination behavior using contact-dependent effectors. Understanding the effects these effectors cause is important to explain bacterial community formation and population dynamics. Typically, kin discrimination effectors are toxins that kill target cells; their effect is therefore obvious ...
    Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions
    斑马鱼细菌感染检测用于研究宿主-病原相互作用
    作者:Faiza Basheer, Clifford Liongue and Alister C Ward日期:03/05/2020,浏览量:274,Q&A: 0
    [Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:323,Q&A: 0
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:341,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Rapid Detection of Proliferative Bacteria by Electrical Stimulation
    利用电刺激快速检测细菌增殖
    作者:Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally日期:02/05/2020,浏览量:830,Q&A: 0
    [Abstract] Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...
    Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
    腺相关病毒样颗粒在杆状病毒系统中的表达纯化及在大肠杆菌AAVR胞外区的构建
    作者:Nancy Meyer, Omar Davulcu, Qing Xie, Mark Silveria, Grant M. Zane, Edward Large and Michael S. Chapman日期:02/05/2020,浏览量:384,Q&A: 0
    [Abstract] Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the outer protein shells (virus-like particles or VLPs) for serotype 2 ...
    Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits
    竞争分析以量化酵母菌对水果上植物病原真菌的生防作用
    作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:378,Q&A: 0
    [Abstract] Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...
    Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
    硫酸酯酶法测定植物对土壤微生物活性的影响
    作者:Anna Koprivova, Achim Schmalenberger and Stanislav Kopriva日期:01/20/2020,浏览量:679,Q&A: 0
    [Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...