微生物学

分类

    现刊
    Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
    菌落计数和活/死染色法评价铜绿假单胞菌生物膜中的活细胞
    作者:Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa日期:09/20/2020,浏览量:83,Q&A: 0
    Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. ...
    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:100,Q&A: 0
    Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...
    Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2
    利用荧光Ca2+指示剂Fura-2测定人类病原体锥虫,墨西哥利什曼原虫和克氏锥虫中细胞内Ca2+的浓度
    作者:Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim日期:09/20/2020,浏览量:70,Q&A: 0
    Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, ...
    Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
    1型单纯疱疹病毒的繁殖,滴定和一步生长曲线
    Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic ...
    Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
    菌落计数和活/死染色法评价铜绿假单胞菌生物膜中的活细胞
    作者:Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa日期:09/20/2020,浏览量:83,Q&A: 0
    [Abstract] Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or ...
    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:100,Q&A: 0
    [Abstract] Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are involved in gene expression regulation. Taken into account the classical gene ...
    Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2
    利用荧光Ca2+指示剂Fura-2测定人类病原体锥虫,墨西哥利什曼原虫和克氏锥虫中细胞内Ca2+的浓度
    作者:Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim日期:09/20/2020,浏览量:70,Q&A: 0
    [Abstract] Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, among many others. This basic premise includes trypanosomatids as Trypanosoma cruzi and various ...
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:729,Q&A: 0
    [Abstract] Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically ...
    Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
    超分辨率共聚焦显微镜活体细胞成像(SCLIM):高时空分辨率的三色、四维同步活体细胞成像
    作者:Kazuo Kurokawa and Akihiko Nakano日期:09/05/2020,浏览量:687,Q&A: 0
    [Abstract] Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo proteins between organelles. For directly visualizing the events in membrane trafficking system, ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    [Abstract] The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and ...
    A High-throughput Interbacterial Competition Platform
    一个高通量细菌间竞争平台
    作者:Hsiao-Han Lin and Erh-Min Lai日期:09/05/2020,浏览量:670,Q&A: 0
    [Abstract] Contact-dependent interbacterial competition is a common strategy used by bacteria to fight for their ecological niches. Interbacterial competition is monitored by a competition assay involving co-culturing the attacker and the recipient bacterial cells on agar, followed by recovery of the surviving recipient cells. Conventional interbacterial ...
    In vitro Cultivation and Visualization of Malaria Liver Stages in Primary Simian Hepatocytes
    猴原代肝细胞体外培养及疟疾肝期观察
    作者:Devendra Kumar Gupta and Thierry Diagana日期:08/20/2020,浏览量:581,Q&A: 0
    [Abstract] Human liver is the primary and obligatory site for malaria infection where sporozoites invade host hepatocytes. Malaria hepatic stages are asymptomatic and represent an attractive target for development of anti-malarial interventions and vaccines. However, owing to lack of robust and reproducible in vitro culture system, it is difficult ...
    Candida albicans Agar Invasion Assays
    白念珠菌琼脂侵入实验
    作者:Shamoon Naseem, Lois M. Douglas and James B. Konopka日期:08/20/2020,浏览量:593,Q&A: 0
    [Abstract] The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can vary at different sites of infection. To identify genes that promote invasive growth, C. albicans ...