Romel Menacho-Melgar Department of Biomedical Engineering, Duke University, Durham, NC
2 protocols

Michael D. Lynch
  • Assistant Professor, Biomedical Engineering, Duke University Durham
Research focus
  • Synthetic Biology, Biomanufacturing, Metabolic Engineering
  • 2 Author merit
2 Protocols published
Measuring Oligonucleotide Hydrolysis in Cellular Lysates via Viscosity Measurements
Authors:  Romel Menacho-Melgar and Michael D. Lynch, date: 01/20/2022, view: 1237, Q&A: 0

Cell lysis, a process that releases host oligonucleotides, is required in many biotechnological applications. However, intact oligonucleotides in crude cellular lysates increase the viscosity of lysates, which complicates downstream processes and

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Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli
Authors:  Romel Menacho-Melgar and Michael D. Lynch, date: 01/20/2022, view: 1191, Q&A: 0

Recombinant protein expression is extensively used in biological research. Despite this, current protein expression and extraction methods are not readily scalable or amenable for high-throughput applications. Optimization of protein expression

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