Prokaryotes

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    Protocols in Current Issue
    EMS Mutagenesis of Clostridium difficile to Identify Strains with Germination-null Phenotypes
    Authors:  Michael B. Francis and Joseph A. Sorg, date: 12/20/2013, view: 10340, Q&A: 0
    [Abstract] Clostridium difficile is a Gram-positive, spore-forming, strict anaerobe and the leading cause of antibiotic-associated diarrhea (McFarland, 2008). Germination by C. difficile spores is the first step in pathogenesis. Thus, identifying mechanisms of C. difficile spore germination may lead to novel anti-germination ...



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