Evolution of Escherichia coli to Macrophage Cell Line
Authors: Migla Miskinyte and
Isabel Gordo,
date: 09/05/2014,
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Q&A: 0
[Abstract] The genomes of species of Escherichia coli (E. coli) show an extraordinary amount of diversity, which include commensal strains and strains belonging to different pathovars. Many strains of E. coli, which can cause mild or severe pathologies in humans, have a commensal ancestor. Understanding the evolutionary changes ...
Fitness Measurements of Evolved Esherichia coli
Authors: Migla Miskinyte and
Isabel Gordo,
date: 09/05/2014,
view: 11030,
Q&A: 0
[Abstract] Bacteria can adapt very rapidly to novel selective pressures. In the transition from commensalism to pathogenicity bacteria have to face and adapt to the host immune system. Specifically, the antagonistic interaction imposed by one of the first line of defense of innate immunity cells, macrophages, on commensal bacteria, such as Escherichia coli ...
Determination of Mutation Frequency During Viral DNA Replication
[Abstract] This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the original Carrascosa et al. (1982), the protocol was carried out with two cloned ...