中文
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Archive
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
About Us
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Cell Biology
Latest Protocol Video
Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
Author Map
Categories
Protocols in Current Issue
By Date
By View
Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry
Authors:
Ana Luque
,
Sonia Cárdenas-Brito
,
Rut Olivar
and
Josep M. Aran
,
date:
11/20/2013,
view:
15922,
Q&A:
0
[Abstract] Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific macropinocytosis (using Lucifer Yelloy CH), and receptor-mediated endocytosis (using DQ
...
Epidermal Growth Factor (EGF) Receptor Endocytosis Assay in A549 Cells
Authors:
Sabrina Rizzolio
and
Luca Tamagnone
,
date:
08/05/2013,
view:
17388,
Q&A:
0
[Abstract] The following endocytosis assay has been optimized to assess EGF-stimulated EGFR endocytosis; but could be modified to assess other ligand-stimulated endocytosis of plasma membrane receptors (for which fluorochrome-conjugated ligands are available to track their receptor internalization). In brief, cells are treated with fluorescent EGF at 4 °C to ...
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.