中文
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Archive
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
About Us
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Prokaryotes
Latest Protocol Video
Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
Author Map
Categories
Protocols in Current Issue
By Date
By View
Generation of Non-typeable
Haemophilus influenzae
Directed Gene Deletion Mutants
Author:
Jeroen D. Langereis
,
date:
03/05/2014,
view:
10000,
Q&A:
0
[Abstract] Directed deletion mutants in non-typeable
Haemophilus influenzae
can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking regions of the target gene. The artificial DNA construct ...
Generation and Screening of a Non-typeable
Haemophilus influenzae
Tn-seq Mutant Library
Author:
Jeroen D. Langereis
,
date:
03/05/2014,
view:
10620,
Q&A:
0
[Abstract] The genome-wide screen Tn-seq (van Opijnen
et al.
, 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.