Protocols in Current Issue
    Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10000, Q&A: 0
    [Abstract] Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking regions of the target gene. The artificial DNA construct ...
    Generation and Screening of a Non-typeable Haemophilus influenzae Tn-seq Mutant Library
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10620, Q&A: 0
    [Abstract] The genome-wide screen Tn-seq (van Opijnen et al., 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...

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