Protocol for the Generation of a Transcription Factor Open Reading Frame Collection (TFome)
Authors: John Gray,
Brett Burdo,
Mary P. Goetting-Minesky,
Bettina Wittler,
Matthew Hunt,
Tai Li,
David Velliquette,
Julie Thomas,
Tina Agarwal,
Kasey Key,
Irene Gentzel,
Michael dos Santos Brito,
Maria Katherine Mejía-Guerra,
Layne N. Connolly,
Dalya Qaisi,
Wei Li,
Maria I. Casas,
Andrea I. Doseff and
Erich Grotewold,
date: 08/05/2015,
view: 13712,
Q&A: 1
[Abstract] The construction of a physical collection of open reading frames (ORFeomes) for genes of any model organism is a useful tool for the exploration of gene function, gene regulation, and protein-protein interaction. Here we describe in detail a protocol that has been used to develop the first collection of transcription factor (TF) and co-regulator ...
Library Construction for Genome-wide Bisulfite Sequencing in Plants
[Abstract] DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. ...