An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo Analysis
Authors: Sara Waise,
Rachel Parker,
Matthew J. J. Rose-Zerilli,
David M. Layfield,
Oliver Wood,
Jonathan West,
Christian H. Ottensmeier,
Gareth J. Thomas and
Christopher J. Hanley,
date: 12/05/2019,
view: 3812,
Q&A: 0
[Abstract] Despite their involvement in many physiological and pathological processes, fibroblasts remain a poorly-characterized cell type. Analysis of primary fibroblasts while maintaining their in vivo phenotype is challenging: standard methods for fibroblast isolation require cell culture in vitro, which is known to alter phenotypes. ...
Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
Authors: Mark Watters and
Eva Szegezdi,
date: 01/05/2017,
view: 7886,
Q&A: 0
[Abstract] The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs (Elliott and Yuan, 2011). In vivo, tumour cells are embedded in a stroma formed primarily by ...
Three Dimensional Spheroid Co-culture Invasion Assay
Authors: Eldo T Verghese and
Thomas A Hughes,
date: 01/05/2015,
view: 11977,
Q&A: 0
[Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...
Three-dimensional Invasion Assay
Authors: Wen-Hao Yang and
Muh-Hwa Yang,
date: 09/05/2013,
view: 11351,
Q&A: 0
[Abstract] The invasive ability of cancer cells is a crucial function for cancer metastasis and the surrounding microenvironment of cancer cells in living tissues is three-dimension (3D). Therefore, to establish an in vitro invasion assay in a 3D system to predict cancer invasive ability is valuable in the research for cancer metastasis. Here, we ...