Biochemistry

Categories

    Protocols in Current Issue
    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    [Abstract]

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. In vitro assays to assess the physical

    ...
    In vitro Auto- and Substrate-Ubiquitination Assays
    Authors:  Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon, date: 04/05/2022, view: 443, Q&A: 0
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

    ...
    Activity-based Crosslinking to Identify Substrates of Thioredoxin-domain Proteins in Malaria Parasites
    Authors:  David W. Cobb, Grace S. Woods and Vasant Muralidharan, date: 02/20/2022, view: 1039, Q&A: 0
    [Abstract] Malaria remains a major public health issue, infecting nearly 220 million people every year. The spread of drug-resistant strains of Plasmodium falciparum around the world threatens the progress made against this disease. Therefore, identifying druggable and essential pathways in P. falciparum parasites remains a major area of ...
    Amber Suppression Technology for Mapping Site-specific Viral-host Protein Interactions in Mammalian Cells
    Authors:  Nur Firdaus Isa, Olivier Bensaude and Shona Murphy, date: 02/05/2022, view: 1109, Q&A: 0
    [Abstract]

    Probing the molecular interactions of viral-host protein complexes to understand pathogenicity is essential in modern virology to help the development of antiviral therapies. Common binding assays, such as co-immunoprecipitation or pull-downs, are helpful in investigating intricate viral-host proteins interactions. However, such assays may miss

    ...
    A Native PAGE Assay for the Biochemical Characterization of G Protein Coupling to GPCRs
    Authors:  Amanda M. Roehrkasse, Jordan A. Karim and Augen A. Pioszak, date: 12/20/2021, view: 2055, Q&A: 0
    [Abstract]

    G protein-coupled receptors (GPCRs) are a large family of membrane-embedded receptors that have diverse roles in physiology and are major drug targets. GPCRs transduce an agonist binding signal across the membrane to activate intracellular heterotrimeric G proteins. The dynamic nature of the receptors and the complexity of their interactions with

    ...
    Label-free Quantification of Direct Protein-protein Interactions with Backscattering Interferometry
    Authors:  Seher Abbas and Karl-Wilhelm Koch, date: 12/20/2021, view: 1282, Q&A: 0
    [Abstract]

    The functional performance of a cell depends on how macromolecules, in particular proteins, come together in a precise orientation, how they assemble into protein complexes and interact with each other. In order to study protein-protein interactions at a molecular level, a variety of methods to investigate these binding processes yield affinity

    ...
    Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
    Authors:  Liping Wang, Gang Yu, Alberto P. Macho and Rosa Lozano-Durán, date: 12/05/2021, view: 1823, Q&A: 0
    [Abstract]

    The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein pull-down, yeast two-hybrid (Y2H), co-immunoprecipitation (co-IP), and bimolecular fluorescence

    ...
    Proximity Dependent Biotin Labelling in Zebrafish for Proteome and Interactome Profiling
    Authors:  Zherui Xiong, Harriet P. Lo, Kerrie-Ann McMahon, Robert G. Parton and Thomas E. Hall, date: 10/05/2021, view: 2392, Q&A: 0
    [Abstract]

    Identification of protein interaction networks is key for understanding intricate biological processes, but mapping such networks is challenging with conventional biochemical methods, especially for weak or transient interactions. Proximity-dependent biotin labelling (BioID) using promiscuous biotin ligases and mass spectrometry (MS)-based

    ...
    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    Authors:  Mark W. Soo and Arneet L. Saltzman, date: 09/20/2021, view: 1125, Q&A: 0
    [Abstract]

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more modifications are a key tool to probe these interactions in pull-down assays with recombinant proteins

    ...
    A Gel-Based Assay for Probing Protein Translocation on dsDNA
    Authors:  Christiane Brugger and Alexandra M. Deaconescu, date: 07/20/2021, view: 2033, Q&A: 0
    [Abstract]

    Protein translocation on DNA represents the key biochemical activity of ssDNA translocases (aka helicases) and dsDNA translocases such as chromatin remodelers. Translocation depends on DNA binding but is a distinct process as it typically involves multiple DNA binding states, which are usually dependent on nucleotide binding/hydrolysis and are

    ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.