Systems Biology


    Protocols in Current Issue
    Detection of Transposable Element Insertion Site Polymorphisms by Sequence-Specific Amplification Polymorphism (SSAP)
    Authors:  Véronique Sarilar, Paulina Martinez Palacios and Karine Alix, date: 03/05/2014, view: 10077, Q&A: 0
    [Abstract] Transposable elements represent a major part of any eukaryotic genomes. Notably in plants they can account for more than 80% of the whole genomic sequence (such as in maize). Due to their mobility across the genome, they can act as mutagens but can also be considered as an important source of genetic diversity. It has been shown that they may be ...
    Generation and Screening of a Non-typeable Haemophilus influenzae Tn-seq Mutant Library
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10628, Q&A: 0
    [Abstract] The genome-wide screen Tn-seq (van Opijnen et al., 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...
    High Resolution Detection of Genetic Changes Associated with Transposons
    Authors:  Beery Yaakov and Khalil Kashkush, date: 06/05/2013, view: 9035, Q&A: 0
    [Abstract] Transposable elements (TEs) are repetitive sequences, capable of inducing genetic mutations through their transpositional activity, or by non-homologous or illegitimate recombination. Because of their similarity and often high copy numbers, examining the effects of mutations caused by TEs in different samples (tissues, individuals, species, etc.) ...
    Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)
    Authors:  Maria Pajunen, Elise Pinta and Mikael Skurnik, date: 08/05/2012, view: 13607, Q&A: 0
    [Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition complex that directs transposon ...

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