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Detection of Transposable Element Insertion Site Polymorphisms by Sequence-Specific Amplification Polymorphism (SSAP)
Authors:
Véronique Sarilar
,
Paulina Martinez Palacios
and
Karine Alix
,
date:
03/05/2014,
view:
10077,
Q&A:
0
[Abstract] Transposable elements represent a major part of any eukaryotic genomes. Notably in plants they can account for more than 80% of the whole genomic sequence (such as in maize). Due to their mobility across the genome, they can act as mutagens but can also be considered as an important source of genetic diversity. It has been shown that they may be ...
Generation and Screening of a Non-typeable
Haemophilus influenzae
Tn-seq Mutant Library
Author:
Jeroen D. Langereis
,
date:
03/05/2014,
view:
10628,
Q&A:
0
[Abstract] The genome-wide screen Tn-seq (van Opijnen
et al.
, 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...
High Resolution Detection of Genetic Changes Associated with Transposons
Authors:
Beery Yaakov
and
Khalil Kashkush
,
date:
06/05/2013,
view:
9035,
Q&A:
0
[Abstract] Transposable elements (TEs) are repetitive sequences, capable of inducing genetic mutations through their transpositional activity, or by non-homologous or illegitimate recombination. Because of their similarity and often high copy numbers, examining the effects of mutations caused by TEs in different samples (tissues, individuals, species, etc.) ...
Construction and Screening of a Transposon Insertion Library of
Yersinia enterocolitica
(YeO3-R1)
Authors:
Maria Pajunen
,
Elise Pinta
and
Mikael Skurnik
,
date:
08/05/2012,
view:
13607,
Q&A:
0
[Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal
in vitro
set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition complex that directs transposon ...
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