Prokaryotes

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    Protocols in Current Issue
    Bacterial Synchronized Transfer Assays in Bone Marrow Derived Macrophages
    Authors:  Sedelia R Dominguez, Tom Kawula and Shaun P Steele, date: 11/20/2019, view: 3504, Q&A: 0
    [Abstract] Merocytophagy (“mero”, Greek for partial; “cytophagy” for cell eating) is a process by which cells acquire microbes and cytosolic material through phagocytosis of a small portion of neighboring cells upon cell-cell contact. Cell-cell contact dependent transfer events can be assessed through co-incubation of differently labeled cells. With these ...
    Generating Isogenic Deletions (Knockouts) in Francisella tularensis, a Highly-infectious and Fastidious Gram-negative Bacterium
    Authors:  Xiaojun Wu, Guoping Ren and Jason F. Huntley, date: 06/20/2015, view: 8829, Q&A: 0
    [Abstract] Generating bacterial gene deletion mutants, also known as knockouts (KOs), is a powerful tool to investigate individual gene functions. However, fastidious bacteria such as Francisella tularensis (F. tularensis) often are difficult to genetically manipulate. Indeed, many different approaches have been tested to generate F. ...



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