Protocols in Current Issue
    GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
    Authors:  Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 11666, Q&A: 0
    [Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...
    Growth Assay for the Stem Parasitic Plants of the Genus Cuscuta
    Authors:  Volker Hegenauer , Max Welz, Max Körner and Markus Albert, date: 04/20/2017, view: 10491, Q&A: 0
    [Abstract] Cuscuta spp. are widespread obligate holoparasitic plants with a broad host spectrum. Rootless Cuscuta penetrates host stems with so called haustoria to form a direct connection to the host vascular tissue (Dawson et al., 1994; Lanini and Kogan, 2005; Kaiser et al., 2015). This connection allows a steady uptake ...
    Forest GPP Calculation Using Sap Flow and Water Use Efficiency Measurements
    Authors:  Fyodor Tatarinov, Eyal Rotenberg, Dan Yakir and Tamir Klein, date: 04/20/2017, view: 7313, Q&A: 0
    [Abstract] This is a protocol to evaluate gross primary productivity (GPP) of a forest stand based on the measurements of tree’s sap flow (SF), 13C derived water use efficiency (WUE), and meteorological (met) data. GPP was calculated from WUE and stomatal conductance (gs), the later obtained from SF up-scaled from sampled trees to stand ...
    Determination of Molecular Structures of Condensed Tannins from Plant Tissues Using HPLC-UV Combined with Thiolysis and MALDI-TOF Mass Spectrometry
    Authors:  Sauro Bianchi, Ivana Kroslakova and Ingo Mayer, date: 10/20/2016, view: 10301, Q&A: 0
    [Abstract] Condensed tannins extracted from plant tissues are suitable substitutes for phenolic resins. Their molecular structure, which might influence their chemical reactivity, can be assessed by the use of both HPLC-UV after acid thiolysis and MALDI-TOF mass spectrometry. Thiolysis of plant extracts in acidic methanol with cysteamine hydrochloride ...
    Sample Preparation for X-ray Micro-computed Tomography of Woody Plant Material and Associated Xylem Visualisation Techniques
    Author:  Nicholas J. B. Brereton, date: 03/20/2016, view: 8646, Q&A: 0
    [Abstract] Variation in the tissue structure of short rotation coppice (SRC) willow is a principle factor driving differences in lignocellulosic sugar yield yet much of the physiology and development of this tissue is unknown. Traditional sectioning can be both difficult and destructive in woody tissue; however, technology such as three dimensional X-ray ...
    An Evaluation of Cellulose Degradation Affected by Dutch Elm Disease
    Authors:  Jaroslav Ďurkovič, František Kačík and Dušan Olčák, date: 07/20/2015, view: 7588, Q&A: 0
    [Abstract] The pathogenic fungus Ophiostoma novo-ulmi spreads within the secondary xylem vessels of infected elm trees, causing the formation of vessel plugs due to tyloses and gels, which ultimately result in Dutch elm disease. Foliage discoloration, wilting and falling from the tree are typical external leaf symptoms of the disease followed by the ...
    Extraction of Nonstructural Carbon and Cellulose from Wood for Radiocarbon Analysis
    [Abstract] This method aims at isolating nonstructural organic carbon (NSC) pools, i.e. soluble sugars and starch, from wood for radiocarbon (14C) analysis at natural abundance levels (≤1 ppt).

    Pools are operationally defined to 1) physically isolate pools - prohibiting the use of destructive methods, such as compound-specific ...

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