Other Vertebrates


    Protocols in Current Issue
    Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue
    [Abstract] Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method ...
    The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells
    Authors:  Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert Langer and Mario P. Tschan, date: 07/05/2019, view: 6647, Q&A: 0
    [Abstract] The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for immuno-histochemical studies. Here we present the CAM assay as a possible model to study autophagy in different types ...
    The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
    Authors:  Piero Crespo and Berta Casar, date: 10/20/2016, view: 19237, Q&A: 0
    [Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary ...
    Chick Neural Tube Explant Culture
    Authors:  Zhanna Alekseenko, Elisabet Andersson and José M. Dias, date: 10/05/2015, view: 9204, Q&A: 0
    [Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor ...
    Electroporation of Embryonic Chick Eyes
    Authors:  Agustín Luz-Madrigal, Erika Grajales-Esquivel and Katia Del Rio-Tsonis, date: 06/20/2015, view: 11938, Q&A: 0
    [Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger and Hamilton, 1951). It originates from ...
    Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons
    Authors:  Nicole H. Wilson and Esther T. Stoeckli, date: 07/05/2014, view: 11665, Q&A: 0
    [Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in vivo model for the identification of ...
    Implantation of Dkk-1-soaked Beads into the Neural Tube of Chicken Embryos
    Authors:  Almudena Martinez-Ferre, Maria Navarro-Garberí, Carlos Bueno and Salvador Martinez, date: 11/05/2013, view: 11301, Q&A: 0
    [Abstract] Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective embryo regions at precise ...
    In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays
    Authors:  Zhenguo Chen, Zhihua Wen and Xiaochun Bai, date: 09/20/2013, view: 35557, Q&A: 3
    [Abstract] Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe an in vivo chick embryo ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.